Question

求准确翻译 谢谢

Rhodamine Labeling of Protein. Three hundred micrograms of purified Pr-2 protein was suspended with 50 mM sodium borate buffer (pH8.5) and mixed with the 5(6)-carboxytetramethylrhodamine N-succimidyl ester solution dissolved in dimethyl sulfoxide (DMSO), and the mixture was then incubated for 2 h at 37 C in the dark. Rhodamine-labeled Pr-2 was purified on C18 RP-HPLC system as the above purification method. Confocal Laser Scanning Microscopy. Confocal laser scanning microscopy was used to analyze the cellular distribution of the protein. Fungal cell suspensions (104 conidia/mL) were poured on poly-L-lysinecoated glass slides, which were subsequently incubated at room temperature (RT) for 45 min to allow the cells to adhere to the slides. Next, after which the slides were washed with PBS, rhodamine-labeled Pr-2 was added. The samples were incubated for 30 min at RT, and the slides were then rinsed several times with PBS. The cells were observed using a confocal laser scanningmicroscope (CLSM, 510META, Zeiss,Gottingen, Germany) (24).

Answer 1

罗丹明标记的蛋白质。三百微克纯化镨- 2蛋白被禁赛50毫米四硼酸钠缓冲液（pH8.5），并与5（6混合）carboxytetramethylrhodamine ñ - succimidyl酯解决方案，二甲基亚砜（DMSO）溶解，当时的混合物培养37 C在黑暗中2小时。罗丹明标记的镨- 2的纯化在C18反相与上述纯化的方法HPLC系统。共聚焦激光扫描显微镜。共聚焦激光扫描显微镜来分析蛋白质的细胞分布。真菌细胞悬浮液（104孢子/毫升）的聚注入基- L - lysinecoated玻璃片，随后在室温下45分钟（往返）培养，使细胞坚持幻灯片。下一步，然后在幻灯片是用PBS，罗丹明标记水洗镨- 2的补充。对样品进行了培养，室温30分钟，而当时的幻灯片用PBS漂洗几次。观察细胞的利用共聚焦激光scanningmicroscope（CLSM，510META，蔡司，哥廷根，德国）（24）。