Question

请高手给我翻译一下 不要在线翻译软件翻译的

The cellular integrity of the hepatocytes in the conventional plates and in the mini bioreactor over the cultivation period was examined by their release of LDH into the medium. The hepatocytes released half the amount of LDH when in the bioreactor compared to the plates between days 6 and 10 (mean level: 2.0 0.4 mU (bioreactor) vs. 4.6 0.6 mU(plate)), starting from 0.17 0.1 mU on day 2 for both systems (Fig. 3). Further cultivation of the heaptocytes in either system led to an elevation of LDH release which was about 1.5 times higher in the bioreactor than in the plates at the end of cultivation (day 17). These findings demonstrate that the bioreactor has no major advantages over the conventional plates regarding the cellular integrity.

Glucose Consumption and Lactate Formation
The aerobic state of the bioreactor and plates was investigated by monitoring glucose consumption and lactate formation by the hepatocytes cultured in both systems. After a decline in the cells’ glucose consumption rates from 720 65 nmol/h on day 2 to 420 136.7 nmol/h for the plates and 155.7 135.6 nmol/h for the bioreactor on day 6, they remained at the mean level of 416 87.8 nmol/h and 78.8 41.5 nmol/h for the plates and bioreactor, respec-tively, during the remaining cultivation period (Fig. 4A). This finding indicates a difference in glucose consumption by the hepatocytes between both systems which was approximately 4.5 times (P <0.0001) lower in the bioreactor than in the plates. Lactate formation by the cells cultured in the plates increased markedly over the cultivation period with a peak on day 13 (260.2 15.1 nmol/h), while a minimal increase of lactate concentration in the bioreactor was observed from day 2 to day 13 (36.4 25.5 nmol/h), demonstrating a seven times (P <0.0001) higher lactate formation by the cells in the plates than in the bioreactor (Fig. 4B). Taken together, these results suggest that the use of a gas-permeable membrane in the bioreactor meets the hepatocytes’ need for oxygen.

Answer 1

在传统板块的肝细胞的完整性和对微型生物反应器培养期间进行了检查，他们的LDH释放到培养基中。肝细胞释放乳酸脱氢酶的一半数量的生物反应器时相比，6至10天的板（平均水平：2.0 0.4亩（生物反应器），与4.6 0.6亩（板）），第2天起，从0.17 0.1亩两个系统（图3）。在两种制度的heaptocytes进一步种植导致的LDH释放了1.5倍，比在板块在培养结束（17日）生物反应器高海拔。这些结果表明，生物反应器方面拥有完整的常规蜂窝板没有大的优势。

葡萄糖消耗和乳酸形成
该板块好氧生物反应器和国家进行了调查监测，在这两个系统培养的肝细胞葡萄糖消耗及乳酸的形成。后，在细胞的葡萄糖消耗率从720下降65 nmol / h的第2天至420 136.7 nmol /对钢板和155.7 135.6 nmol水平/天的生物反应器6小时，他们停留在平均水平416 87.8 nmol / h和78.8 41.5 nmol /小时的盘子和生物反应器，respec - tively，在余下的培育期（图4A）之一。这一发现表明，在葡萄糖消耗两个系统之间的约4.5倍（P“0.0001肝细胞的差额）的比板反应器低。乳酸板块在培养的细胞的形成显着增加用13天（260.2 15.1 nmol种植高峰期/小时），而在反应器乳酸浓度最小有所增加，从第2天至13天（36.4 25.5 nmol /小时），显示了7倍（P“0.0001）高比在生物反应器（图4b）板块的细胞乳酸的形成。总而言之，这些结果表明，使用的气体在渗透膜生物反应器符合肝细胞'对氧气的需要。