Question

求大神帮忙翻译一段和生物有关的文字 感谢

Viewed fromthe top, the structure contains a central hydrophobic ring of 76A ° in diameter (Fig. 4a, left panel) with a 32A ° pore in the middle. This region consists of a ring of two-helix bundles (Fig. 4a, lower right panel) formed by helices a2 and a3 of the TraFCT antenna. These helices each forma ring, with the a3 ring inside the a2 ring, suggesting that a2 is the transmembrane helix contacting the membrane. Two lines of evidence indicate that this region is inserted in the outer membrane and forms the outer-membrane channel. First, a2, the external helix in the two-helix bundle, has all the features of a transmembrane
helix: it is amphipathic, with its hydrophobic side expected to contact the membrane, and is strongly predicted to form a transmembrane helix by TMPred . Second, when a Flag-tag is inserted between
helices a2 and a3 of TraF in the full-length core complex, the tag is found exposed extracellularly (Fig. 4b), demonstrating that the twohelix bundle projects across the outer membrane. Altogether, these results unambiguously assign the two-helix bundle region of TraF/VirB10 as the channel-forming region of T4S systems.

Answer 1

从顶上看，该结构包含直径76年°中央疏水环（图4A，左面板）中间有一个32A°孔。这个区域包括一个环的两个螺旋束（图4A，右下方）的trafct A2和A3形成螺旋天线。这些螺旋型环，环内的A2 A3环，这表明A2是跨膜螺旋接触膜。两行的证据表明，这个区域是插在外膜和外膜通道的形成。首先，A2，在两个螺旋束外螺旋，具有所有的跨膜的特点
螺旋：它是两性的，其疏水性侧将接触的膜，并强烈预测的跨膜螺旋结构形成。第二，当一个标签之间插入
在A2和A3全长核心复杂螺旋TRAF，标签被发现暴露在细胞外（图4B），表明twohelix束工程横跨外膜。总之，这些结果明确地分配流量/ VirB10两个螺旋束区域作为沟道形成区4s系统。