Question

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Wet biomass of R. sphaeroides in fermentative medium
was collected according to the following procedure.
Fermented mash was centrifuged at 10,000 rpm for
20 min. The sediment was collected and washed twice with
distilled water, then placed in open beakers and dried in a
Labconco freeze-drier (Free Zone) for 48 h at 50 1C and
pressure o1 Pa.

Answer 1

在发酵性介质中的R. sphaeroides 潮湿生物质，按照以下的流程收集。已发酵的饲料在10,000 rpm （每分钟1万转儿）的条件下用离心机分离20分钟。沉淀物被收集并用蒸馏水清洗两次，然后放在开口的烧杯里并在Labconco冷冻干燥机里干燥，条件是温度50 1度，压力o1 Pa，时间为48小时。