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鸭L1CAM基因CDS区全长为3516bp,编码由1171个氨基酸组成、相对分子量为129772.1Da,等电点为5.98的不稳定亲水蛋白,该蛋白含有信号肽,具有五个跨膜...
鸭L1CAM基因CDS区全长为3516 bp,编码由1171个氨基酸组成、相对分子量为129772.1Da,等电点为5.98的不稳定亲水蛋白,该蛋白含有信号肽,具有五个跨膜区,分别位于该氨基酸序列的0~29、254~277、424~442、827~847和1045~1063区域,同源性比对表明该基因核苷酸序列与鸭与原鸡、虎皮鹦鹉、鸽、斑胸草雀分别具有91.6%、91.4%、90.4%和86.5%的一致性,功能位点分析显示它含有91个活性位点,该氨基酸序列位点59~78,133~148,245~258,477~507,630~648,669~680,730~762,866~886和1074~1098区域可能是抗原表位。
本研究对这两个基因结构的分析,为进一步了解这两个基因的功能奠定了基础。 展开
本研究对这两个基因结构的分析,为进一步了解这两个基因的功能奠定了基础。 展开
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The full-length CDS region of duck L1CAM gene is 3516 BP, encoding 1171 amino acids, the molecular weight is 129772.1Da, isoelectric point is not stable hydrophilic protein 5.98, the protein contains a signal peptide, five transmembrane regions, are located in the amino acid sequence of 0~29, 254~277, 424~442, 827~847 and 1045~1063 regions, homologous alignment showed that the consistency of the gene sequence and duck and fowl, parrots, pigeons, zebra finches have respectively 91.6%, 91.4%, 90.4% and 86.5%, sequence analysis showed it contains 91 active sites, the amino acid sequence of loci 59~78133~148245~258477~507630~648669~680730~762866~886 and 1074~1098 regions may be epitope. In this study, analysis of the two gene structure, laid the foundation for the further understanding of the function of two genes.
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The full length of the CDS domain of duck LICAM gene is 3516 bp, the code consists of 1171 amino acids, with a relative molecular weight of 129772.1Da, which is an instable hydrophilic protein with an isoelectric point of 5.98. The protein contains a signal peptide, may be localized in the endoplasmic reticulum, containing the signal peptide, having five transmembrane domains, which are located in the domains of 0 to 29, 254 to 277, 424 to 442, 827 to 847 and 1045~1063, respectively; the homologue comparison shows that the nucleotide sequence derived from that gene has the consistency of 91.6%, 91.4%, 90.4% and 86.5% with original chicken, budgies, pigeons, zebra finch, respectively; and the functional site analysis shows that it contains 91 active sites, the sites of the amino acid sequence 59~78,133~148, 245~258, 477~507, 630~648, 669~680, 730~762, 866~886 and 1074~1098 domains may be the epitopes.
The present research analyzes these two gene structures, and lays a foundation for the knowledge of these two genes.
The full length of the CDS domain of duck LICAM gene is 3516 bp, the code consists of 1171 amino acids, with a relative molecular weight of 129772.1Da, which is an instable hydrophilic protein with an isoelectric point of 5.98. The protein contains a signal peptide, may be localized in the endoplasmic reticulum, containing the signal peptide, having five transmembrane domains, which are located in the domains of 0 to 29, 254 to 277, 424 to 442, 827 to 847 and 1045~1063, respectively; the homologue comparison shows that the nucleotide sequence derived from that gene has the consistency of 91.6%, 91.4%, 90.4% and 86.5% with original chicken, budgies, pigeons, zebra finch, respectively; and the functional site analysis shows that it contains 91 active sites, the sites of the amino acid sequence 59~78,133~148, 245~258, 477~507, 630~648, 669~680, 730~762, 866~886 and 1074~1098 domains may be the epitopes.
The present research analyzes these two gene structures, and lays a foundation for the knowledge of these two genes.
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