Question

求翻译~~~~~~~~~~~

目的：制备、纯化N-甲基D-门冬氨酸受体（NMDAR1）的单克隆抗体。方法：把NMDAR1杂交瘤细胞注射到小鼠制备抗体，yong ProteinA-Sepharose CL-4B亲和层析纯化单。用Western—blot鉴定该抗体特异性。结果：Western—blot鉴定证实在大鼠脑组织可以明确识别到NMDAR1抗体。结论 纯化了的抗体，可以用来进行NMDAR的进一步研究

Answer 1

Objective: the preparation and purification N - methyl D - nmda receptor (NMDAR1) monoclonal antibodies. Methods: the NMDAR1 ZaJiaoLiu cell injections into mice yong, ProteinA - antibody preparation Sepharose CL - 4B affinity chromatography purification. In Western blot -- the antibody specificity. Results: the Western blot -- JianDingZheng rats can truly explicitly identify to NMDAR1 organization. Conclusion the purification of antibody, can be used for the NMDAR further study

用有道翻的，不知道行不行，水平有限啊，不要拿砖拍我啊拍我啊，呵呵

Answer 2

Objective: preparation and purification of anti-NMDAR1(N-methyl, D-NMDA receptor) monoclonal antibody. Methods: NMDAR1 hybridoma cell was injected into mice and then was purified using affinity chromatography with Protein A-Sepharose CL-4B. The specificity of anti-NMDAR1 monoclonal antibody was confermed by western blotting. Result: anti-NMDAR1 monoclonal antibody was obviously identified in the brain tissue from injected-mice by western blotting. Conclusion: anti-NMDAR1 monoclonal antibody was purified in current study and it would be used in further research.
这是什么论文摘要啊？？ 自己翻译的，水平不高，慎用~~~呵呵

Answer 3

Objective: To prepare and purify the monoclonal antibodies of NMDAR1.

Method: Inject the hybridoma cells of NMDAR1 into the prepared antibodies of mice, purify it with Protein A-Sepharose CL-4B affinity chromatography, and identify the specificity of the antibodies with Western—blot.

Result: The Western—blot identification confirmed that NMDAR1 antibodies can be clearly distinguished in the brain tissues of the mice.

Conclusion: Purified antibodies can be applied to conduct further research.

【英语牛人团】

Answer 4

你是广工制药的？？