Question

求英文高手帮我翻译下，变态老师给了一篇英文专业论文，有关DNA基因转到担子菌中~~

DNA transformations were performed as described, each time using 1 μg of the trp1+
plasmid pCc1001 for selection and, where required, 1 μg of a plasmid of the pYSK series.
Transformed protoplasts were plated on regeneration agar plates supplemented with 0.5 mM ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)]. Transformants were picked for further growth onto minimal medium.
Selected transformants were inoculated in the middle of the YMG/T agar plates
(Ø 9 cm) containing 0.5 mM ABTS and grown at room temperature. Daily increases in growth radii and the extension of a red-brown zone caused by a laccase-mediated oxidation of ABTS were marked. To test enzymatic activities in shaken liquid culture (120 rpm, 37°C), transformants were grown in 500-ml Erlenmeyer flasks in 100 ml of YMG/T liquid medium. In some experiments,CuSO4 was added to the medium in concentrations of 0.1 and 1.0 mM, respectively. Cultures were inoculated with 7 ml of suspended mycelium obtained from the homogenization of a 5-day-old YMG/T agar preculture in 50 ml of sterile YMG/T.

Answer 1

DNA的转移像以上所述，每次使用1μg trp1+质粒用于选择，以及1μgpYSK的序列。
转化过的原生质体接种在再生胶上，补充0.5 mM 的ABTS [2,2′-次偶氮基-反(3-乙基苯并噻唑-6-磺酸)。挑选出转化子再基本培养基上继续培养。
将选出的转化子接种于YMGT/T胶的中央，胶中含有0.5 mM的ABTS，室温培养。多酚氧化酶介导的ABTS氧化能够标记菌斑每日生长的大小。为了测定摇床培养(120 rpm, 37°C)液体培养基中酶的活性，转化子在500ml锥形瓶中培养，加入100ml的YMG/T液体培养基。在一些实验中，在培养基中分别加入0.1和1.0mM的硫酸铜。然后培养基中再加入7ml来自于在无菌YMG/T胶中预培养了5天的悬浮菌丝。

我承认我无聊了。。。

Answer 2

这也太专业了吧...

Answer 3

DNA的转换进行了描述，每次使用1微克+ trp1
质粒的选择，并在有需要时，1系列的质粒的pYSK微克pCc1001。
转化原生质体再生的培养基上镀板辅以0.5毫米ABTS [2,2'- azino -二（3 -乙基- 6 -磺酸）]。挑取转化为基本培养基上进一步增长。
选定的转化子在YMG /吨琼脂板中接种
（直径9厘米）含0.5毫米ABTS和室温生长。每日增加增长半径和由ABTS漆酶介导的氧化引起的红褐色区域扩展了标记。要测试动摇液体培养酶的活性（120转，37℃），转化生长在500毫升锥形瓶在100 YMG /吨液体培养基毫升。在一些实验中，硫酸铜添加到培养基中的0.1和1.0毫米，分别为浓度。文化接种菌丝体悬浮7从一个5天大的YMG /吨琼脂预培养同质化取得50无菌YMG /吨毫升毫升.