Question

谁有加拿大哥伦比亚大学设计并公布的ISSR引物序列啊，能发一份给我么 geatone@163.com。谢谢！

谁有加拿大哥伦比亚大学设计并公布的ISSR引物序列啊，能发一份给我么
geatone@163.com。谢谢！

Answer 1

UBC Primer Set #9 (Microsatellite)
引物名称 序列
801 ATA TAT ATA TAT ATA TT
802 ATA TAT ATA TAT ATA TG
803 ATA TAT ATA TAT ATA TC
804 TAT ATA TAT ATA TAT AA
805 TAT ATA TAT ATA TAT AC
806 TAT ATA TAT ATA TAT AG
807 AGA GAG AGA GAG AGA GT
808 AGA GAG AGA GAG AGA GC
809 AGA GAG AGA GAG AGA GG
810 GAG AGA GAG AGA GAG AT
811 GAG AGA GAG AGA GAG AC
812 GAG AGA GAG AGA GAG AA
813 CTC TCT CTC TCT CTC TT
814 CTC TCT CTC TCT CTC TA
815 CTC TCT CTC TCT CTC TG
816 CAC ACA CAC ACA CAC AT
817 CAC ACA CAC ACA CAC AA
818 CAC ACA CAC ACA CAC AG
819 GTG TGT GTG TGT GTG TA
820 GTG TGT GTG TGT GTG TC
821 GTG TGT GTG TGT GTG TT
822 TCT CTC TCT CTC TCT CA
823 TCT CTC TCT CTC TCT CC
824 TCT CTC TCT CTC TCT CG
825 ACA CAC ACA CAC ACA CT
826 ACA CAC ACA CAC ACA CC
827 ACA CAC ACA CAC ACA CG
828 TGT GTG TGT GTG TGT GA
829 TGT GTG TGT GTG TGT GC
830 TGT GTG TGT GTG TGT GG
831 ATA TAT ATA TAT ATA TYA
832 ATA TAT ATA TAT ATA TYC
833 ATA TAT ATA TAT ATA TYG
834 AGA GAG AGA GAG AGA GYT
835 AGA GAG AGA GAG AGA GYC
836 AGA GAG AGA GAG AGA GYA
837 TAT ATA TAT ATA TAT ART
838 TAT ATA TAT ATA TAT ARC
839 TAT ATA TAT ATA TAT ARG
840 GAG AGA GAG AGA GAG AYT
841 GAG AGA GAG AGA GAG AYC
842 GAG AGA GAG AGA GAG AYG
843 CTC TCT CTC TCT CTC TRA
844 CTC TCT CTC TCT CTC TRC
845 CTC TCT CTC TCT CTC TRG
846 CAC ACA CAC ACA CAC ART
847 CAC ACA CAC ACA CAC ARC
848 CAC ACA CAC ACA CAC ARG
849 GTG TGT GTG TGT GTG TYA
850 GTG TGT GTG TGT GTG TYC
851 GTG TGT GTG TGT GTG TYG
852 TCT CTC TCT CTC TCT CRA
853 TCT CTC TCT CTC TCT CRT
854 TCT CTC TCT CTC TCT CRG
855 ACA CAC ACA CAC ACA CYT
856 ACA CAC ACA CAC ACA CYA
857 ACA CAC ACA CAC ACA CYG
858 TGT GTG TGT GTG TGT GRT
859 TGT GTG TGT GTG TGT GRC
860 TGT GTG TGT GTG TGT GRA
861 ACC ACC ACC ACC ACC ACC
862 AGC AGC AGC AGC AGC AGC
863 AGT AGT AGT AGT AGT AGT
864 ATG ATG ATG ATG ATG ATG
865 CCG CCG CCG CCG CCG CCG
866 CTC CTC CTC CTC CTC CTC
867 GGC GGC GGC GGC GGC GGC
868 GAA GAA GAA GAA GAA GAA
869 GTT GTT GTT GTT GTT GTT
870 TGC TGC TGC TGC TGC TGC
871 TAT TAT TAT TAT TAT TAT
872 GAT AGA TAG ATA GAT A
873 GAC AGA CAG ACA GAC A
874 CCC TCC CTC CCT CCC T
875 CTA GCT AGC TAG CTA G
876 GAT AGA TAG ACA GAC A
877 TGC ATG CAT GCA TGC A
878 GGA TGG ATG GAT GGA T
879 CTT CAC TTC ACT TCA
880 GGA GAG GAG AGG AGA
881 GGG TGG GGT GGG GTG
882 VBV ATA TAT ATA TAT AT
883 BVB TAT ATA TAT ATA TA
884 HBH AGA GAG AGA GAG AG
885 BHB GAG AGA GAG AGA GA
886 VDV CTC TCT CTC TCT CT
887 DVD TCT CTC TCT CTC TC
888 BDB CAC ACA CAC ACA CA
889 DBD ACA CAC ACA CAC AC
890 VHV GTG TGT GTG TGT GT
891 HVH TGT GTG TGT GTG TG
892 TAG ATC TGA TAT CTG AAT TCC C
893 NNN NNN NNN NNN NNN
894 TGG TAG CTC TTG ATC ANN NNN
895 AGA GTT GGT AGC TCT TGA TC
896 AGG TCG CGG CCG CNN NNN NAT G
897 CCG ACT CGA GNN NNN NAT GTG G
898 GAT CAA GCT TNN NNN NAT GTG G
899 CAT GGT GTT GGT CAT TGT TCC A
900 ACT TCC CCA CAG GTT AAC ACA

其中：N = (A , G, C , T) , R = (A , G) ,Y = (C , T) ,B = (C , G, T) ( I. e. not A) ,D = (A , G, T)
( I. e. not C) ,H = (A , C , T) ( I. e. not G) ,V = (A , C , G) ( I. e. not T)

转自 http://zhidao.baidu.com/question/36329992.html?fr=ala0 by fengfeixue0219