Question

求翻译 不要谷歌哈。

All isolates with MICs of ciprofloxacin of at least 4 μg per milliliter were serotyped by the National Reference Center for Streptococcus (Edmonton, Alta.) with the use of type-specific antiserum.19 The genomic DNA of two groups of 12 and 14 randomly chosen isolates with MICs of ciprofloxacin of 1 μg per milliliter and 2 μg per milliliter, respectively, as well as all isolates with MICs of ciprofloxacin of at least 4 μg per milliliter, were examined by pulsed-field gel electrophoresis after digestion with SmaI in order to clarify clonal relationships.20,21 These isolates were further characterized by polymerase-chain-reaction and restriction-fragment–length polymorphism analysis with HinfI for the most commonly recognized mutations in parC and gyrA associated with resistance to fluoroquinolones that have previously been described in Staphylococcus aureus and Escherichia coli. 22,23

Answer 1

人工翻译，喜欢加分：

所有达到4微克/毫升环丙氟哌酸最小抑菌浓度（MIC）的分离菌株都由国家链球菌参考中心（埃德蒙顿，艾伯塔省）通过特定类型抗血清进行了血清分型（按表面抗原分类）。

两组分别为12个和14个随机选择的分离菌株——其MIC分别为1微克/毫升和2微克/毫升环丙氟哌酸，以及所有4微克/毫升环丙氟哌酸MIC的分离株，都在Smal内切酶消化处理后通过脉冲场凝胶电泳法（PFGE）测定了其染色体DNA，以便阐明其间的纯系关系。

通过多聚酶链式反应以及Hinfl（一种限制酶）限制性片段长度多态性分析，这些菌株被进一步按照通常存在于金黄色葡萄球菌及大肠杆菌中的parC和gyrA基因片段抗氟喹诺酮类药物变异进行了区分。

Answer 2

所有的分离环丙沙星和中等收入国家至少4μg是为每毫升血清中心由国家参考链球菌(阿尔塔省埃德蒙顿)使用抗血清.19类型详细而精确的基因组DNA的两组12至14个随机选择隔离环丙沙星和中等收入国家中,有1和2μg为每毫升μg分别为每毫升、环丙沙星和中等收入国家分离至少4μg检查每毫升,pulsed-field凝胶电泳SmaI消化后,为了查明,21岁的克隆人际关系.20这些隔离聚合酶链特征,进一步分析,为HinfI多态性最普遍公认的突变gyrA帕洛阿尔托研究中心和耐氟喹诺酮类药物相关进行了此前,这些金黄色葡萄球菌和大肠杆菌22、23