Question

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（1）VEGF在NS、LS及SH中均无表达；在VIN中表达于外阴真皮组织中的散在细胞内，细胞质呈棕黄色颗粒，阳性率为35%（7/20），明显高于NS、SH和LS，其差异有统计学意义（P＜0.05）；在VSCC中表达在肿瘤细胞的细胞质内，呈棕褐色，着色深且阳性细胞较为集中，阳性率为80%（16/20），明显高于正常外阴皮肤组织、SH、LS及VIN，其差异有统计学意义（P＜0.05）。（2）CD34蛋白阳性表达定位于血管内皮细胞胞浆或胞膜，在新生的微血管及非新生血管包括大血管的内皮细胞上均呈阳性表达，呈棕黄色至棕褐色颗粒。可较好的反映出外阴组织真皮内的MVD情况。在正常外阴皮肤组织中MVD值为20.80，在SH中MVD值为29.45，在LS中MVD值为17.40，在VIN中MVD值为38.90，在VSCC中MVD值为46.85，各病变组织与正常外阴皮肤组织相比均有显著差异（P＜0.05），且各组之间也具有显著的差异性（P＜0.05）。
实验结论 : 1. 本研究结果显示VEGF在正常外阴组织、LS及SH中均无表达，说明在正常外阴皮肤组织、LS和SH中血管新生水平均较低；VEGF在VIN中呈弱表达，在VSCC中呈强表达，进一步说明了VEGF促进了VIN和VSCC新血管生成，在外阴组织癌变的过程中起到了重要作用。 2.LS中的MVD值较正常皮肤低，说明LS中存在微循环障碍；SH中的MVD值较正常皮肤明显增多，说明SH中微循环较正常丰富，可促进SH的发生；VIN的MVD值比正常外阴组织和NNEDV要高，但是比VSCC要低，说明VIN上皮的异型增生较良性增殖（如SH）真皮内的血运更为丰富，血流量增多，可能加速异型细胞增长，甚至癌变；VSCC中的MVD值最高，说明在VSCC中微循环丰富，可促进肿瘤的生长。3. 本研究结果表明VEGF高表达者，MVD亦高表达，进一步证实了VEGF与血管生成的密切关系。

Answer 1

VEGF in NS, LS and SH There was no expression; VIN in the genital expression in dermal tissues of scattered cells within the cytoplasm was brown granules, The positive rate was 35% (papillary hemorrhage), which was significantly higher than that of NS, and SH LS, the difference was statistically significant (P <0.05); the pathways expressed in the cytoplasm of tumor cells. was SEPIA, coloring deep-positive cells more concentrated and the positive rate was 80% (16/20) significantly higher than normal genital skin, SH, LS and VIN, the difference was statistically significant (P <0.05). (2) CD34 protein was localized in the cytoplasm of endothelial cells or membrane. Freshmen in the microvascular and non-angiogenesis including vascular endothelial cells were positive expression Brown was yellow-brown colored granules. May better reflect the genital organizations within the dermis of the MVD. In normal genital skin tissue MVD to 20.80, SH MVD value of 29.45. LS MVD in the value of 17.40, the VIN MVD value of 38.90. MVD pathways in the value of 46.85. the lesions and normal genital skin tissue were significantly different (P <0.05) and the group is also a significant difference (P <0.05). Experimental conclusions : 1. The results of this study showed that VEGF in normal genital organizations, LS and SH There was no expression Note the normal genital skin, LS and SH angiogenesis lower average water; VEGF in the VIN was slightly expression pathways in China showed strong expression further illustrate the VEGF promoted the VIN and new pathways of angiogenesis, Vulvar cancer organizations in the process played an important role. 2.LS the MVD lower than normal skin on LS exist microcirculation; SH of MVD than normal skin noticeably increased, and SH note Microcirculation abundant than normal, SH may promote the occurrence of the MVD ;VIN than normal genital and NNEDV higher But less than carp, Note the VIN epithelial dysplasia more benign proliferative (SH) dermis more abundant blood supply, blood flow increased, abnormal cells could accelerate growth, or even cancer ;VSCC the highest MVD, Note pathways in the micro-circulation and promote tumor growth. 3. The results of this study show high expression of VEGF, MVD high expression further confirmed by VEGF and angiogenesis close relationship. Question : bydlxj-two probationary Magicians
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