Question

求英文翻译成中文 急！！！

We prepared proficiency testing samples with estradiol concentrations ranging from 20 to 600 ng/L. 1 Specimens were distributed to 430 laboratories enrolled in the endocrinology proficiency testing program; 159 of these submitted results for estradiol testing. The mean CV for all methods was 8.4% at estradiol concentrations >100 ng/L. Greater imprecision (CV = 33–40%; n = 16) at estradiol concentrations ranging from 150 to 400 ng/L was observed for the (AxSYM) MEIA method in the proficiency testing event of September 2001. Results from this method were bimodal in distribution, with apparent centers at 120 and 240 ng/L. 2
We examined the possibility that components of the proficiency testing specimens interfered with the AxSYM method. Steroid components of the endocrinology proficiency testing specimens are listed in Table 1 . We examined their potential to interfere with the AxSYM method by adding estradiol to processed human serum (proficiency testing serum matrix) to a final concentration of 1000 ng/L, together with one of the steroids listed in Table 1 at either of two concentrations (one within and one above the reference intervals). Estradiol in each sample was measured by the AxSYM method in an undiluted sample and a 1:5 automated dilution. (Abbott Laboratories has instructed users of the AxSYM instrument to perform a 1:5 automatic dilution of samples for some specimens.) Of the steroids tested, only unconjugated estriol showed an inhibitory effect on estradiol measurements, in the undiluted specimen. The magnitude of this effect was substantially reduced when the assay was performed with the 1:5 automated dilution protocol.

Answer 1

我们准备用雌二醇浓度水平测试样本范围从20至600 ng / l . 1标本被分配到430实验室参与这项内分泌学能力试验程序;159这些提交的成果,为雌二醇测试。平均的简历,所有的方法是在雌二醇浓度> 8.4% 100 ng / L。更大的不精确33-40% CV =(n = 16)、雌二醇浓度范围从150 - 400 ng / L(AxSYM观察的方法)矿山能力试验的事件在2001年9月。结果该方法明显的双峰分布,与中心240 ng / l 120,2

我们检验了这一可能性,零件的熟练鉴定标本妨碍了AxSYM方法。类固醇零件的内分泌学熟练鉴定标本是列于表1。我们检查了他们的潜在干扰了AxSYM加工方法,通过增加人血清雌二醇(能力试验血清基质)到最终的浓度的1000 ng / L,連同类固醇列于表1两种浓度(只有在和一个以上的参考间隔)。雌二醇每个标本中的测量方法的AxSYM稀释样品和一1:5自动稀释。(雅培已经命令的用户AxSYM仪器履行1:5自动稀释的样本,进行一些标本。)类固醇激素测试,只有unconjugated雌三醇表现出抑制作用,在纯精油雌二醇测量标本。这一效果实在的幅度大大减少的情况下进行的检测时的书1:5自动化稀释协议

Answer 2

我们准备用雌二醇浓度水平测试样本范围从20至600 ng / l . 1标本被分配到430实验室参与这项内分泌学能力试验程序;159这些提交的成果,为雌二醇测试。平均的简历,所有的方法是在雌二醇浓度> 8.4% 100 ng / L。更大的不精确33-40% CV =(n = 16)、雌二醇浓度范围从150 - 400 ng / L(AxSYM观察的方法)矿山能力试验的事件在2001年9月。结果该方法明显的双峰分布,与中心240 ng / l 120,2

我们检验了这一可能性,零件的熟练鉴定标本妨碍了AxSYM方法。类固醇零件的内分泌学熟练鉴定标本是列于表1。我们检查了他们的潜在干扰了AxSYM加工方法,通过增加人血清雌二醇(能力试验血清基质)到最终的浓度的1000 ng / L,連同类固醇列于表1两种浓度(只有在和一个以上的参考间隔)。雌二醇每个标本中的测量方法的AxSYM稀释样品和一1:5自动稀释。(雅培已经命令的用户AxSYM仪器履行1:5自动稀释的样本,进行一些标本。)类固醇激素测试,只有unconjugated雌三醇表现出抑制作用,在纯精油雌二醇测量标本。这一效果实在的幅度大大减少的情况下进行的检测时的书1:5自动化稀释协议