
英语高手帮忙,帮忙翻译论文英文摘要,小弟在此谢过啊,不要翻译软件啊
本课题通过采用3,5一二硝基水杨酸比色法(DNS比色法)测定α-淀粉酶的酶活性,之后计算出分配系数K,从而得知聚乙二醇/硫酸铵体系在双水相体系中对α-淀粉酶萃取分离的效果...
本课题通过采用3,5一二硝基水杨酸比色法(DNS比色法)测定α-淀粉酶的酶活性 ,之后计算出分配系数K,从而得知聚乙二醇/硫酸铵体系在双水相体系中对α-淀粉酶萃取分离的效果,通过实验研究考查聚乙二醇的分子量,硫酸铵的浓度,pH值,氯化钠质量分数,体系温度等因素对分配系数的影响,从而得到最佳的萃取分离条件。研究结果表明:双水相体系质量组成为聚乙二醇400026%,硫酸铵21%,氯化钠0.002%,pH5.0时α-淀粉酶的萃取效果较好,实验中分配系数可达3.30。
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请先确认一下:倒数第二行的“聚乙二醇400026%”是什么意思?中间有没有遗漏一些空格或字符?莫非你说的双水相体系中,聚乙二醇的质量必须是总质量的4000。26倍???
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聚乙二醇400026%的意思是聚乙二醇的相对分子质量为4000,质量分数浓度为26%
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The aim of this project is: determine the enzymatic activity of alpha amylase with 3.5-Dinitrosalicylic acid colorimetric method (DNS method), then figure out the distribution coefficient K, finally obtain the effect of alpha amylase extraction separation by polyethylene glycol / ammonium sulfate system in the aqueous two-phase system. Through experimental study examines the affection to the distribution coefficient K by the molecular weight of polyethylene glycol, the concentration of ammonium sulfate, pH value, sodium chloride mass fraction, system temperature etc... Thus obtain the best conditions for extraction separation. The research results indicate that alpha amylase extraction effect is better when the mass component of the aqueous two-phase system to be 26% of polyethylene glycol 4000, 21% of ammonium sulfate, 0.002% of sodium chloride, meanwhile pH equal to 5.0 , so the distribution coefficient K can reach up to 3.30.
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