Question

哪位英语高手帮我翻译一下 谢谢

Analysis. OD600 was determined by spectrophotometry (Spectronic 20+, Milton Roy) at a wavelength of 600 nm. Moreover, bacterial suspensions were centrifuged at 3600 · g for 20 minutes to allow for measurement of ammonia and nitrite levels in the supernatant before assay was performed. Ammonia concentration was then determined by Nessler assay at a wavelength of 425 nm with a sensitivity forammonia detection of 400 lg N L)1 [5]. Nitrite concentration was determined by colorimetry at a wavelength of 543 nm [5]. To determine O2 and N2, 20-lL samples (injection volume) were removed from the headspace of the serum bottles using a pressure-lock syringe (Supelco) and analyzed by GC–TCD [19]. To measure N2O, 500-lL gas samples were removed from the headspace of the serum bottles, once again using a pressure-lock syringe (Supelco) and analyzed by GC with an electron-capture detector (PerkinElmer) [21]. O2, N2, and N2O were quantified by the methods proposed by Su et al. [19, 21].

Answer 1

分析。液的OD 600测定分光光度法（spectronic20 +，密尔顿罗伊）在波长600纳米。此外，细菌悬液，离心3600·克20分钟，使测量氨和亚硝酸盐含量在上清法之前进行。氨浓度测定比色法在波长为425纳米的敏感性forammonia检测400LG的）1[ 5]。亚硝酸盐浓度测定比色法在波长为543纳米的[ 5]。确定氧气和氮气，20-ll样品（注射量）被从顶的血清瓶使用pressure-lock注射器（炉）的气相色谱分析–中药[ 19]。衡量一氧化二氮，500-ll气体样品从顶的血清瓶，再次使用pressure-lock注射器（炉）和分析的气相色谱电子捕获检测器（技术）[ 21]。氧气，氮气，和一氧化二氮的量化的方法提出苏等。[ 19，21 ]。

Answer 2

哎，搞基础的孩子真可怜，我试试看，有些专有名词翻译的不好请见谅。
分析：600nm波长下用光谱仪测量OD600。将细菌悬浮液在3000转/分的转速下离心20min，用以后面测量上清液中的氨和亚硝酸盐的水平。
425nm波长下用Nessler分析法测量氨的浓度，精确度可达400ug/L。
543nm波长下采用比色法测量亚硝酸盐的浓度。
用带压力锁的注射器从血清瓶顶端空间中抽出20IL样本的氧气与氮气，用GC-TCD法分析。
后面就差不多了，不翻了