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卵巢早衰是严重影响妇女身心健康和生活质量的疾病,发病年龄提前,发病率上升,激素替代疗法存在风险。本课题根据肾主生殖及月经周期阴阳消长规律,提出遵循月经周期,在经净后用中药...
卵巢早衰是严重影响妇女身心健康和生活质量的疾病,发病年龄提前,发病率上升,激素替代疗法存在风险。本课题根据肾主生殖及月经周期阴阳消长规律,提出遵循月经周期,在经净后用中药先补肾阴、后补肾阳的类似于西医的雌孕激素序贯疗法,前期临床治疗卵巢早衰疗效显著。为了探明该法治疗卵巢早衰的机理,以经典名方左归丸、右归丸为实验用药,以《内经》“任脉通,太冲脉盛,月事以时下”理论为切入点,以反应卵巢新生血管的VEGF和提示卵巢储备水平的INHB为观察点,根据阴道细胞学变化,选择自然初老雌鼠为卵巢早衰模型,分模型组、中药组、西药组、中西药结合组,遵循大鼠性周期从动情间期第二日开始先用左归丸、后用右归丸,连续药物干预8个周期,观察卵巢组织VEGF的表达和血清INHB的变化,结合基础窦卵泡数、卵巢体积及性激素变化,判断该治法对卵巢血流和储备功能的影响,为肾主生殖及防治卵巢早衰提供实验依据。
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Ovarian premature aging is seriously affect women's physical and mental health and quality of life of the disease, early age of onset, the rise in incidence rates, hormone replacement therapy is at risk. This topic according to the kidneys reproductive and menstrual cycles change rules of Yin and Yang, put forward the following menstrual cycle, and the net with Chinese traditional medicine in after the first newly-risen point after the kidney Yang, similar to western medicine al.sex hormone sequential therapy, the clinical treatment of ovarian premature aging curative effect is distinct. For the treatment of ovarian proved premature mechanism, with classical name then be pills, pills for the study drug to the right, with the theory of "ren tong, pulse emmeniopathy fill,YueShi to nowadays "theory as the breakthrough point, with reaction ovarian new blood vessels VEGF and hint ovarian reserve level for the INHB condensation, according to the vaginal cytology change, choose the natural old for premature ovarian early female model, points model group, Chinese traditional medicine group, western medicine group, the traditional combination group, follow the rats from sexual cycle between the second day of estrogen started use first left to pills, with right after to pills, continuous drug intervention eight cycle, observation ovarian tissue VEGF expression and serum INHB change, combining basic sinus follicular number, ovarian volume and sex hormone changes, if this comes to the ovary blood flow and reserve the influence of function, for the kidneys and prevention and control premature ovarian reproductive to provide the basis.
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Premature ovarian failure is a serious impact on women's physical and mental health and quality of life, disease, early age of onset, increased incidence of hormone replacement therapy risk. Population fluctuation of this topic based on the Kidneys reproductive and menstrual cycles of yin and yang, proposed to follow the menstrual cycle, with traditional Chinese medicine through the net first kidney yin and kidney yang sequential therapy of estrogen and progesterone similar to Western medicine, pre-clinical treatment of premature ovarian failure a significant effect. Proven for the treatment of premature ovarian failure mechanism to the left of the classic side Gui Wan, YouguiWan experimental drug Neijing "Ren, the Taichong pulse Sheng menstruation nowadays" theory as the starting point ovarian neovascular response of VEGF and prompt ovarian reserve level INHB for the observation point, according to the changes in vaginal cytology, natural early old female premature ovarian failure model, divided into model group, the Medicines and western medicine group, combined with Western medicine group, Follow rat sexual cycle from the second day of diestrus first ZuoguiWan after use YouguiWan, eight cycles of continuous drug intervention, to observe the expression of VEGF in ovarian tissue and serum INHB changes, combined with the basis of the number of antral follicles, ovarian volume and hormonal changes, to determine the impact on ovarian blood flow and functional reserve of the governing law, to provide the experimental basis for the Kidneys reproductive and prevention of premature ovarian failure.
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