Question

麻烦大家帮我翻译一下，急求要专业一点的

tumor in the T2 weighted MR image, while the unlabeled
MCF-7 cells did not show any fluorescence signal and MR
contrast enhancement in both images (Figure S7 in the Sup-
porting Information).
It is well-known that nanoparticles can accumulate at tumors
via the enhanced permeation and retention (EPR) effect, which
results from extravasation into leaky vascular endothelial cells
around tumors.
17
Passive targeting and accumulation of Fe3O4-MSN at the tumor site could be demonstrated by in vivo MR
imaging. Fe3O4-MSN was injected intravenously into a nude
mouse bearing a tumor on its shoulder.
T2 weighted MR images at 1.5 T were obtained before and
after the injection. At 3 h after injection, a drop in the MR signal
was detected at the tumor site, demonstrating passive targeting
of Fe3O4-MSN caused by the EPR effect (Figure 4a). To confirm
accumulation of Fe3O4-MSN, the mouse was sacrificed 24 h
after the injection, and its tumor tissue was sectioned and
observed by CLSM. The accumulation of Fe3O4-MSN at the
tumor site was verified by orange RITC fluorescence (Figure
4b). The MR and CLSM images showed that the Fe3O4-MSN
also accumulated in the liver, spleen, and lung via phagocytosis
of macrophages (Figures S8, S9 in the Supporting Informa-
tion).
18
Delivery of drug to tumor site was demonstrated with Fe3O4-MSN synthesized without fluorescent dye. Nude mice bearing
a tumor were given intravenous injections of free Fe3O4-MSN
and DOX loaded Fe3O4-MSN. For DOX loaded Fe3O4-MSN,
injection doses of DOX were 2 and 4 mg/kg. The mice were
sacrificed 48 h after the injection, and their tumor tissues were
sectioned and observed by CLSM. Red fluorescence of DOX
allowed direct visualization of drug accumulation at the tumor
site of mice treated with DOX loaded Fe3O4-MSN, while red
fluorescence was hardly detected at tumor site of mouse treated
with free Fe 3O4-MSN (Figure S10 in the Supporting Informa-tion). To examine antitumor activity of the drug, apoptotic and
nonapoptotic cells in tumor tissues were evaluated using terminal
deoxynucleotidyl transferase-mediated nick end labeling (TUNEL)
assay (Figure 5). TUNEL-positive tumor cell nuclei (brown
color) with apoptotic morphology were detected in tumor tissues
of mice treated with DOX loaded Fe3O4-MSN (Figure 5b,c),
whereas apoptotic cells were rarely detectable in the case ofree Fe3O4-MSN (Figure5a). These results show that DOX was
delivered to the tumor site successfully and its antitumor activity
was retaine

Answer 1

肿瘤在T 2加权图像，而标签

MCF - 7细胞并没有显示任何荧光和核磁共振信号

对比增强图像（图7支援—

移植的信息）。

众所周知，纳米粒子可以积聚在肿瘤

通过加强渗透和保留（乙丙橡胶）的影响，其中

结果从外渗到漏血管内皮细胞

周围肿瘤。

17

被动靶向和积累fe3o4-msn在肿瘤部位可以证明体内磁共振

成像。fe3o4-msn静脉注射到裸鼠

小鼠肿瘤其肩上。

T 2加权磁共振图像获得了之前和在1.5吨

注射后。在注射后3小时，下降的信号

检测在肿瘤部位，表明被动靶向

fe3o4-msn所造成的电子顺磁共振的影响（图4）。确认

积累fe3o4-msn，鼠标24

注射后，与肿瘤组织切片和

通过激光共聚焦显微镜观察。积累fe3o4-msn在

肿瘤网站验证了橙色瑞达国贸荧光（图

4 B）。磁共振和激光共聚焦显微镜图像显示，fe3o4-msn

也积累的肝，脾，肺通过吞噬

巨噬细胞（图8，9在支持信息—

等）。

18

运送毒品的肿瘤的网站被证明与fe3o4-msn无荧光染料的合成。裸鼠

肿瘤被给予静脉注射游离fe3o4-msn

与阿霉素加载fe3o4-msn。阿霉素加载fe3o4-msn，

注射剂量的阿霉素的2和4毫克/千克。小鼠

牺牲后48小时注射，和他们的肿瘤组织

切片，观察激光共聚焦显微镜。红色荧光的阿霉素

允许直接可视化的药物积累在肿瘤

现场处理小鼠阿霉素加载fe3o4-msn，而红

荧光是很难检测到在肿瘤部位处理小鼠

游离铁3o4-msn（图组织在支持信息）。研究抗肿瘤活性的药物，细胞凋亡和

性细胞在肿瘤组织进行了评价使用终端

脱氧核苷酸转移酶介导的末端标记（法）

法（图5）。TUNEL阳性肿瘤细胞的细胞核（棕色

彩色）与细胞凋亡形态被发现在肿瘤组织

治疗小鼠阿霉素加载fe3o4-msn（图5二，三），

而细胞凋亡很少被探测到的情况ofree fe3o4-msn（figure5a）。这些结果表明，阿霉素是

传递到肿瘤部位成功及其抗肿瘤活性

Answer 2

医学领域, 要向专业团队求助.

Answer 3

这个还真看不懂，，寻求答案……

追问

等我知道我告诉你啊