Question

麻烦你们帮我翻译一下 急求

tumor in the T2 weighted MR image, while the unlabeled
MCF-7 cells did not show any fluorescence signal and MR
contrast enhancement in both images (Figure S7 in the Sup-
porting Information).
It is well-known that nanoparticles can accumulate at tumors
via the enhanced permeation and retention (EPR) effect, which
results from extravasation into leaky vascular endothelial cells
around tumors.
17
Passive targeting and accumulation of Fe3O4-MSN at the tumor site could be demonstrated by in vivo MR
imaging. Fe3O4-MSN was injected intravenously into a nude
mouse bearing a tumor on its shoulder.
T2 weighted MR images at 1.5 T were obtained before and
after the injection. At 3 h after injection, a drop in the MR signal
was detected at the tumor site, demonstrating passive targeting
of Fe3O4-MSN caused by the EPR effect (Figure 4a). To confirm
accumulation of Fe3O4-MSN, the mouse was sacrificed 24 h
after the injection, and its tumor tissue was sectioned and
observed by CLSM. The accumulation of Fe3O4-MSN at the
tumor site was verified by orange RITC fluorescence (Figure
4b). The MR and CLSM images showed that the Fe3O4-MSN
also accumulated in the liver, spleen, and lung via phagocytosis
of macrophages (Figures S8, S9 in the Supporting Informa-
tion).
18
Delivery of drug to tumor site was demonstrated with Fe3O4-MSN synthesized without fluorescent dye. Nude mice bearing
a tumor were given intravenous injections of free Fe3O4-MSN
and DOX loaded Fe3O4-MSN. For DOX loaded Fe3O4-MSN,
injection doses of DOX were 2 and 4 mg/kg. The mice were
sacrificed 48 h after the injection, and their tumor tissues were
sectioned and observed by CLSM. Red fluorescence of DOX
allowed direct visualization of drug accumulation at the tumor
site of mice treated with DOX loaded Fe3O4-MSN, while red
fluorescence was hardly detected at tumor site of mouse treated
with free Fe 3O4-MSN (Figure S10 in the Supporting Informa-tion). To examine antitumor activity of the drug, apoptotic and
nonapoptotic cells in tumor tissues were evaluated using terminal
deoxynucleotidyl transferase-mediated nick end labeling (TUNEL)
assay (Figure 5). TUNEL-positive tumor cell nuclei (brown
color) with apoptotic morphology were detected in tumor tissues
of mice treated with DOX loaded Fe3O4-MSN (Figure 5b,c),
whereas apoptotic cells were rarely detectable in the case ofree Fe3O4-MSN (Figure5a). These results show that DOX was
delivered to the tumor site successfully and its antitumor activity
was retaine

Answer 1

肿瘤在T2加权图像先生,而unlabeled
MCF-7细胞并没有表现出任何的荧光信号,先生
在两张图像对比度增强(图S7在见sub -
将信息)。
众所周知,纳米粒子可以累积在肿瘤
通过加强渗透和保留(EPR)效果,哪一个
结果在血管内皮细胞外漏
在肿瘤。
17
被动靶向性和累积的Fe3O4-MSN肿瘤网站可能会在体内了先生
成像。Fe3O4-MSN静脉内注射成一个裸体
老鼠肩负着一个肿瘤。
T2加权磁共振影像在1.5吨的前取得
在注入。在注射后3小时以内,降幅在磁共振信号
在检测到肿瘤部位、论证被动靶向吗
Fe3O4-MSN EPR的造成的效果(图4)。 确认
Fe3O4-MSN积累,老鼠牺牲了24小时
注射后,其肿瘤组织和区段
观察到CLSM。在Fe3O4-MSN的积累
肿瘤的位置进行了验证RITC荧光橙色(图
4 b)。先生,Fe3O4-MSN CLSM图像显示
也积累了在肝脏、脾脏、肺脏通过吞噬
巨噬细胞(数据,并与S9 S8支援信息-
小轿车)。
18
交付的药物与肿瘤部位被证明没有Fe3O4-MSN合成萤光染料。裸鼠轴承
给出了一个肿瘤的自由Fe3O4-MSN静脉注射
“Fe3O4-MSN和加载。 “Fe3O4-MSN为加载,
注射剂量的“2和4毫克/公斤。“老鼠
牺牲了48小时注射后,他们的肿瘤组织
分段和遵守CLSM。红色荧光的“
允许直接可视化的药物在肿瘤积累
网站“治疗组小鼠Fe3O4-MSN加载,而红色的
荧光几乎没有检测到肿瘤部位的老鼠对待
以自由铁三O4-MSN(图S10支援信息)。检查抗肿瘤活性的药物,凋亡
nonapoptotic细胞的肿瘤组织中使用终端进行评估
deoxynucleotidyl核尼克原位末端标记(TUNEL)
分析法(图5)。TUNEL-positive肿瘤细胞核(棕色
颜色)与凋亡形态学检测肿瘤组织中
“治疗组小鼠装载Fe3O4-MSN(图5 b,c),
而检测细胞凋亡的情况很少ofree Fe3O4-MSN(Figure5a)。 “这些研究结果表明
成功送达肿瘤的位置和它的抗肿瘤活性
是retaine

Answer 2

肿瘤在T2加权图像先生,而unlabeled
　　MCF-7细胞并没有表现出任何的荧光信号,先生
　　在两张图像对比度增强(图S7在见sub -
　　将信息)。
　　众所周知,纳米粒子可以累积在肿瘤
　　通过加强渗透和保留(EPR)效果,哪一个
　　结果在血管内皮细胞外漏
　　在肿瘤。
　　17
　　被动靶向性和累积的Fe3O4-MSN肿瘤网站可能会在体内了先生