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本论文以水稻红良166号为材料，通过施加外源NO供体硝普钠 (sodiumnitroprusside，SNP)和NO清除剂cPTIO，确定在50 μmol·L-1 Al3+的胁迫下，SNP和cPTIO缓解水稻铝毒的最佳处理浓度和处理时间。结果表明，铝抑制水稻根系生长，SNP预处理显著提高根相对伸长率，减少根尖Al含量，且在0.1 mmol·L-1SNP处理效果最好。 cPTIO逆转SNP对水稻铝毒的缓解作用，且在0.075 mmol·L-1能够明显消减外源NO。SNP和cPTIO对水稻铝毒的作用均在6h时效果最好。因此，确定SNP和cPTIO预处理的最佳浓度分别为0.1 mmol·L-1和0.075 mmol·L-1，处理时间为6h。

Answer 1

本论文以水稻红良166号为材料，通过施加外源NO供体硝普钠 (sodiumnitroprusside，SNP)和NO清除剂cPTIO，确定在50 μmol•L-1 Al3+的胁迫下，SNP和cPTIO缓解水稻铝毒的最佳处理浓度和处理时间。

This dissertation attempts to determine the optimum concentration and treatment time of sodium nitropprus-side (SNP) and cPTIO for the remission of aluminum toxicity in rice (Oryza sativa L.) under the stress of 50 μmol•L-1 Al3+, by using the Hongliang No.166 rice as a sample material, and by treating with the exogenous nitric oxide donor, SNP and scavenger agent, cPTIO.

结果表明，铝抑制水稻根系生长，SNP预处理显著提高根相对伸长率，减少根尖Al含量，且在0.1 mmol•L-1SNP处理效果最好。 cPTIO逆转SNP对水稻铝毒的缓解作用，且在0.075 mmol•L-1能够明显消减外源NO。SNP和cPTIO对水稻铝毒的作用均在6h时效果最好。

The result shows that aluminum inhibits the root growth in rice, but with the pretreatment of SNP, the relative elongation rate of the roots is significantly enhanced, the Al content at the root tips reduced and the best treatment effect is achieved with a concentration of 0.1 mmol•L-1 SNP. cPTIO reverses SNP’s remission effect on the aluminum toxicity in rice; the Al toxicity effect in rice can be best reduced by the exogenous nitric oxide donor, SNP and cPTIO with a concentration of 0.075 mmol•L-1, and the optimum duration is 6 hours.

因此，确定SNP和cPTIO预处理的最佳浓度分别为0.1 mmol•L-1和0.075 mmol•L-1，处理时间为6h。

Therefore, the best effective concentration rate for the pretreatment of SNP and cPTIO is 0.1 mmol•L-1 and 0.075 mmol•L-1 respectively, and the optimum treatment time is 6 hours.

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