Question

求助，帮忙翻译一篇摘要，汉译英，谢谢！

本研究选取转录调节因子Slr0895作为目的基因，从集胞藻Synechocystis sp. PCC 6803 中提取基因组，通过PCR扩增得到目的基因上下游片段作为同源臂构建基因敲除所需片段，在两个同源片段中间插入氯霉素（chloramphenicol，简称Cm）抗性基因片段，借助琼脂糖电泳来验证融合是否成功。由于集胞藻Synechocystis sp. PCC 6803 有天然的吸收外源DNA的能力，利用这一特性将融合好的突变片段转入集胞藻Synechocystis sp. PCC 6803 细胞中，在含有氯霉素的抗性平板上培养数天直至长出单菌落，再进行菌落PCR来快速鉴定目的基因是否得到成功敲除。将突变株与野生型进行比较，观察其表型。

Answer 1

This study selected transcription factor Slr0895as gene, from Synechocystis sp. PCC 6803Synechocystis genome by gene, amplified by PCR downstream fragments as homologous arm knockout constructs required fragment, in two homologous fragment is inserted into the middle of chloramphenicol ( chloramphenicol, Cm) resistance gene fragment, with the help of agarose gel electrophoresis to verify whether successful fusion. As a result of Synechocystis Synechocystis sp. PCC 6803is the natural absorption of exogenous DNA ability, use this property to the fused the mutant fragment into Synechocystis Synechocystis sp. PCC 6803 cells, in containing chloramphenicol resistance plate cultured for several days until a single colony, colony PCR again to rapid identification of target genes is success knockout. The mutant and wild type were compared, to observe its phenotype.

Answer 2

This study selected transcription factor Slr0895 as gene, from Synechocystis sp. PCC 6803 Synechocystis genome by gene, amplified by PCR downstream fragments as homologous arm knockout constructs required fragment, in two homologous fragment is inserted into the middle of chloramphenicol (chloramphenicol, Cm) resistance gene fragment, with the help of agarose gel electrophoresis to verify whether successful fusion.As a result of Synechocystis Synechocystis sp. PCC 6803 is the natural absorption of exogenous DNA ability, use this property to the fused the mutant fragment into Synechocystis Synechocystis sp. PCC 6803 cells, in containing chloramphenicol resistance plate cultured for several days until a single colony, colony PCR again to rapid identification of target genes is success knockout.The mutant and wild type were compared, to observe its phenotype.