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3.3.Invivotest3.3.1.D.polymorphaSomepreviousstudieshavereportedthatmusseltolerancetop...
3.3. In vivo test
3.3.1. D. polymorpha
Some previous studies have reported that mussel tolerance to
pollutants is regulated by seasonal changes (Van Benschoten et
al., 1995) and by temperature (Harrington et al., 1997; Buschini
et al., 2003a), since temperature-dependent metabolic and filtration
rates (Quigley et al., 1993; Kilgour and Baker, 1994) can
modulate exposure to toxicants.
In this study, the data obtained by the comet assay on haemocytes
of zebra mussels directly collected in the lake water during
the three different seasonal periods (Table 3I) were not significantly
different. Comparable results were obtained for all the
samplings in different seasons, both at the start of observation
period and 20 days after, when the total length of DNA
migration in the comet assay was considered. The great interindividual
variability could affect the results and mask eventual
seasonal differences. The use of the ratio between the migration
length and the diameter of the comet head (LDR, see Tice et
al., 2000) as a parameter to represent the data of the genotoxic
effects in D. polymorpha was found (Bolognesi et al., 2004)
to be able to reduce the inter-individual variability of the data
(from 20–30% for TL to 5–10% for LDR). When considering
LDR (Table 3II), our results are partially in agreement with literature
data (Quigley et al., 1993; Kilgour and Baker, 1994; Van
Benschoten et al., 1995; Harrington et al., 1997; Buschini et al.,
2003a). A lower DNA damage was found during the coldest
period (time 0; p = 0.02, winter versus both autumn and summer).
However, in winter, a significant increase (p < 0.001) of
baseline DNA breakage is detected in the second sampling (20
days later).
Table 展开
3.3.1. D. polymorpha
Some previous studies have reported that mussel tolerance to
pollutants is regulated by seasonal changes (Van Benschoten et
al., 1995) and by temperature (Harrington et al., 1997; Buschini
et al., 2003a), since temperature-dependent metabolic and filtration
rates (Quigley et al., 1993; Kilgour and Baker, 1994) can
modulate exposure to toxicants.
In this study, the data obtained by the comet assay on haemocytes
of zebra mussels directly collected in the lake water during
the three different seasonal periods (Table 3I) were not significantly
different. Comparable results were obtained for all the
samplings in different seasons, both at the start of observation
period and 20 days after, when the total length of DNA
migration in the comet assay was considered. The great interindividual
variability could affect the results and mask eventual
seasonal differences. The use of the ratio between the migration
length and the diameter of the comet head (LDR, see Tice et
al., 2000) as a parameter to represent the data of the genotoxic
effects in D. polymorpha was found (Bolognesi et al., 2004)
to be able to reduce the inter-individual variability of the data
(from 20–30% for TL to 5–10% for LDR). When considering
LDR (Table 3II), our results are partially in agreement with literature
data (Quigley et al., 1993; Kilgour and Baker, 1994; Van
Benschoten et al., 1995; Harrington et al., 1997; Buschini et al.,
2003a). A lower DNA damage was found during the coldest
period (time 0; p = 0.02, winter versus both autumn and summer).
However, in winter, a significant increase (p < 0.001) of
baseline DNA breakage is detected in the second sampling (20
days later).
Table 展开
1个回答
2008-03-07
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3.3 。在体内试验
3.3.1 。四多形
先前的一些研究报告说,淡菜耐受
污染物是调节季节性变化(范benschoten等
基地, 1995年)和温度(哈林顿等人, 1997年;布斯基尼
等人, 2003年) ,因为温度依赖性代谢和过滤
费率(奎格利等人, 1993年; kilgour和贝克, 1994年) ,可
抑扬接触毒物。
在这项研究中,所获得的资料是由彗星试验对血细胞
斑马贻贝直接收集在湖水中
三个不同的季节期(表特殊国与国关系)都没有显着
不同的。比较的结果,获得了所有
采样,在不同的季节,不论是在一开始的观察
期及20天之后,当总长度的DNA
叠前偏移技术在彗星试验进行了审议。伟大interindividual
变异可能影响结果,并最终面具
季节性差别。使用的,两者的比例约为迁移
长度和直径的彗星头(低剂量辐射,见泰斯对持不同等
人, 2000年)作为参数,以代表该数据的遗传毒性
效应四多形被发现( bolognesi等人, 2004年)
为了能够减少跨个别变异性数据
(从20-30 %铊,以5-10 %的LDR ) 。当考虑
低剂量辐射(表3ii ) ,我们的结果是,部分协议与文学
资料(奎格利等人, 1993年; kilgour和贝克, 1994年;货车
benschoten等人, 1995年哈林顿等人, 1997年;布斯基尼等人,
2003A点) 。较低的DNA损伤被发现,在最寒冷
期间(时间0 ; P值0.02 ,冬季银两都秋季和夏季) 。
不过,在冬天,显着升高( P < 0.001 )
基线DNA断裂,是在发现第二次采样( 20
几天后) 。
表
3.3.1 。四多形
先前的一些研究报告说,淡菜耐受
污染物是调节季节性变化(范benschoten等
基地, 1995年)和温度(哈林顿等人, 1997年;布斯基尼
等人, 2003年) ,因为温度依赖性代谢和过滤
费率(奎格利等人, 1993年; kilgour和贝克, 1994年) ,可
抑扬接触毒物。
在这项研究中,所获得的资料是由彗星试验对血细胞
斑马贻贝直接收集在湖水中
三个不同的季节期(表特殊国与国关系)都没有显着
不同的。比较的结果,获得了所有
采样,在不同的季节,不论是在一开始的观察
期及20天之后,当总长度的DNA
叠前偏移技术在彗星试验进行了审议。伟大interindividual
变异可能影响结果,并最终面具
季节性差别。使用的,两者的比例约为迁移
长度和直径的彗星头(低剂量辐射,见泰斯对持不同等
人, 2000年)作为参数,以代表该数据的遗传毒性
效应四多形被发现( bolognesi等人, 2004年)
为了能够减少跨个别变异性数据
(从20-30 %铊,以5-10 %的LDR ) 。当考虑
低剂量辐射(表3ii ) ,我们的结果是,部分协议与文学
资料(奎格利等人, 1993年; kilgour和贝克, 1994年;货车
benschoten等人, 1995年哈林顿等人, 1997年;布斯基尼等人,
2003A点) 。较低的DNA损伤被发现,在最寒冷
期间(时间0 ; P值0.02 ,冬季银两都秋季和夏季) 。
不过,在冬天,显着升高( P < 0.001 )
基线DNA断裂,是在发现第二次采样( 20
几天后) 。
表

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