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全文的结构安排如下：第一部分，人民币汇率制度变化过程，以及中国在各个阶段的汇率制度的变迁，再分析近五年人民币明显升值阶段；第二部分，人民币升值的原因。从多方面论证为什么人...
全文的结构安排如下：第一部分，人民币汇率制度变化过程，以及中国在各个阶段的汇率制度的变迁，再分析近五年人民币明显升值阶段；第二部分，人民币升值的原因。从多方面论证为什么人民币有升值的现象；第三部分，人民币升值对外贸的影响。这部分详细分析了中国人民币升值给中国外贸带来那些有利或不利的影响；第四部分，人民币升值的相应的对策。针对人民币升值给中国外贸带来的压力，本文提出了一些解决措施，以便缓解这些压力。最后是本文的结论部分。
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Hepatitis E virus IgG antibody diagnostic kit
(Enzyme-linked immunosorbent assay) Manual
[Name]: Hepatitis E virus IgG antibody diagnostic kit (enzyme-linked immunosorbent assay)
[Generic name: The hepatitis E virus IgG antibody diagnostic kit (enzyme-linked immunosorbent assay)
[Name]:
[Name]: Anti-HEV IgG ELISA Kit
[Packaging specifications: The 96 people were / box; 48 people were / box;
[Expected] uses: qualitative detection in human serum or plasma hepatitis E virus IgG antibody
Principle [test]
The kit used genetic engineering recombinant expression of high-grade, high-activity HEV virus-specific antigen (ORF2, ORF3) coated ELISA plates, horseradish peroxidase (HRP) labeled anti-mouse IgG (r chain) Monoclonal antibody tracer, TMB color system, indirect ELISA for detection in human serum or plasma in the hepatitis E virus IgG antibodies, applicable to the diagnosis of hepatitis E and hepatitis E virus infection of the epidemiological investigation.
[Kit comprising:
Kit packaging specifications 96T/48T
1, measured by the HEV-IgG-board 96-/ 48 -
2, on a diluted specimen bottles (12 ml/6ml)
3, of a combination of bottles (12 ml/6ml)
4, washing liquid (20 x) 1 bottle (50 ml/25ml)
5, a bottle of liquid substrate (6 ml/3ml)
6, the termination of a bottle (6 ml/3ml)
7, a bottle of negative control (0.5 ml/0.3ml)
8, a bottle of positive control (0.5 ml/0.3ml)
9, in a statement
10, a Plastic Bag
11, formwork two closures
[Specimen collection and preservation]
By conventional methods serum samples collected from the vein. Plasma samples can be conventional dosage of anticoagulant heparin or sodium citrate. Determination within five days of the specimens can be placed in the preservation of -20 ℃ at least three months. Specimens avoid hemolysis or repeated freeze solution. Turbid or sediment samples should be clarified after centrifugation or filtration detection. The serum must be kept in the collection, preservation should pay attention to the process of aseptic operation.
[Steps]
1, for a blank hole, without any liquids (2 hole for each of the negative control, 2 Kongyang of control). Diluted liquid samples from 100 μ l Add reaction hole, using sample of the 3rd Army blending more than 37 ℃ for 30 minutes.
2, washed with distilled water will be diluted by 20 times after plate washer. Abandoning a previous rejection reaction solution, in each hole by adding diluted washing liquid 300 μ l, still 30 seconds. Rejection of disposable washing liquid, so repeatedly washed five times, the last in the shoot dry absorbent paper.
3, each hole by adding enzyme conjugate 100 μ l, and 37 ℃ for 30 minutes.
4, plate washer five times, with two steps.
5, followed by adding at the end of each hole of A, B of the 50 μ l, 37 ℃ dark color for 10 minutes.
6, the termination of each hole by adding 50 μ l, blending termination reaction. Determination within 30 minutes OD value.
[Results] found
Meibiaoyi a wavelength of 450. Stay in the gaps with air-conditioning, and then read the hole OD value.
Critical value (Cutoff) = 0.10 + average OD value of the negative control (negative control average OD value <0.05 to 0.05)
Determination specimens OD value <critical value for the resistance - HEV-IgG negative.
Determination specimens OD value ≥ critical value for the resistance - HEV-IgG positive.
[Notes]
1, 2-8 ℃ home kit stored. Detection may not be repeated freezing and thawing samples, or not long hemolytic bacteria.
2, reagents Do not mix different batches! Yao Jun various reagents used before; if washing liquid crystal precipitation, Yao Jun (or mild heating) does not affect the use of dissolved.
3, in strict accordance with the Manual operation, with special attention to strict hole system reaction time and reaction temperature. Kit home to the former balance of 30-60 minutes at room temperature.
4, the reaction can not be a board Kaifeng exhausted, and the remaining slab at the same time Add desiccant closures Plastic bags, and home 2-8 ℃, can be short-term preservation.
5, incubated, will be closed to plate affixed to the membrane reaction board to prevent the entry of foreign matter or droplets reaction hole. Closure plate membrane can not reused.
6, all specimens, waste, according to the positive control, such as transmission of treatment (control has been inactivated serum treatment).
Approval Number: Duration: 12 months
请采纳答案，支持我一下。
(Enzyme-linked immunosorbent assay) Manual
[Name]: Hepatitis E virus IgG antibody diagnostic kit (enzyme-linked immunosorbent assay)
[Generic name: The hepatitis E virus IgG antibody diagnostic kit (enzyme-linked immunosorbent assay)
[Name]:
[Name]: Anti-HEV IgG ELISA Kit
[Packaging specifications: The 96 people were / box; 48 people were / box;
[Expected] uses: qualitative detection in human serum or plasma hepatitis E virus IgG antibody
Principle [test]
The kit used genetic engineering recombinant expression of high-grade, high-activity HEV virus-specific antigen (ORF2, ORF3) coated ELISA plates, horseradish peroxidase (HRP) labeled anti-mouse IgG (r chain) Monoclonal antibody tracer, TMB color system, indirect ELISA for detection in human serum or plasma in the hepatitis E virus IgG antibodies, applicable to the diagnosis of hepatitis E and hepatitis E virus infection of the epidemiological investigation.
[Kit comprising:
Kit packaging specifications 96T/48T
1, measured by the HEV-IgG-board 96-/ 48 -
2, on a diluted specimen bottles (12 ml/6ml)
3, of a combination of bottles (12 ml/6ml)
4, washing liquid (20 x) 1 bottle (50 ml/25ml)
5, a bottle of liquid substrate (6 ml/3ml)
6, the termination of a bottle (6 ml/3ml)
7, a bottle of negative control (0.5 ml/0.3ml)
8, a bottle of positive control (0.5 ml/0.3ml)
9, in a statement
10, a Plastic Bag
11, formwork two closures
[Specimen collection and preservation]
By conventional methods serum samples collected from the vein. Plasma samples can be conventional dosage of anticoagulant heparin or sodium citrate. Determination within five days of the specimens can be placed in the preservation of -20 ℃ at least three months. Specimens avoid hemolysis or repeated freeze solution. Turbid or sediment samples should be clarified after centrifugation or filtration detection. The serum must be kept in the collection, preservation should pay attention to the process of aseptic operation.
[Steps]
1, for a blank hole, without any liquids (2 hole for each of the negative control, 2 Kongyang of control). Diluted liquid samples from 100 μ l Add reaction hole, using sample of the 3rd Army blending more than 37 ℃ for 30 minutes.
2, washed with distilled water will be diluted by 20 times after plate washer. Abandoning a previous rejection reaction solution, in each hole by adding diluted washing liquid 300 μ l, still 30 seconds. Rejection of disposable washing liquid, so repeatedly washed five times, the last in the shoot dry absorbent paper.
3, each hole by adding enzyme conjugate 100 μ l, and 37 ℃ for 30 minutes.
4, plate washer five times, with two steps.
5, followed by adding at the end of each hole of A, B of the 50 μ l, 37 ℃ dark color for 10 minutes.
6, the termination of each hole by adding 50 μ l, blending termination reaction. Determination within 30 minutes OD value.
[Results] found
Meibiaoyi a wavelength of 450. Stay in the gaps with air-conditioning, and then read the hole OD value.
Critical value (Cutoff) = 0.10 + average OD value of the negative control (negative control average OD value <0.05 to 0.05)
Determination specimens OD value <critical value for the resistance - HEV-IgG negative.
Determination specimens OD value ≥ critical value for the resistance - HEV-IgG positive.
[Notes]
1, 2-8 ℃ home kit stored. Detection may not be repeated freezing and thawing samples, or not long hemolytic bacteria.
2, reagents Do not mix different batches! Yao Jun various reagents used before; if washing liquid crystal precipitation, Yao Jun (or mild heating) does not affect the use of dissolved.
3, in strict accordance with the Manual operation, with special attention to strict hole system reaction time and reaction temperature. Kit home to the former balance of 30-60 minutes at room temperature.
4, the reaction can not be a board Kaifeng exhausted, and the remaining slab at the same time Add desiccant closures Plastic bags, and home 2-8 ℃, can be short-term preservation.
5, incubated, will be closed to plate affixed to the membrane reaction board to prevent the entry of foreign matter or droplets reaction hole. Closure plate membrane can not reused.
6, all specimens, waste, according to the positive control, such as transmission of treatment (control has been inactivated serum treatment).
Approval Number: Duration: 12 months
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