Question

论文摘要 英译汉 ~~给很多分很多分噢！！

Abstract:
To isolate anti-salt stress genes from mangrove plants, a cDNA library of R. stylosa roots was constructed and screened for stress-related genes by polymerase chain reaction (PCR)- based suppressive subtractive hybridization (SSH). The mangrove seedlings were grown for 8 months under two conditions: exposure to water with 3% salt and exposure to fresh water. cDNA of seedlings exposed to water with 3% salt was used as a tester and cDNA for freshwater germination was used as a driver. We isolated and sequenced 240 up-regulated expressed sequence tags (ESTs) from the SSH library. Among these up-regulated ESTs, 48 unique clones were putatively identified and classified into ten functional categories, such as cell rescue and defense, secondary metabolism, protein synthesis, and metabolism. Fifteen genes from different categories were selected and their expression was studied by real-time RT-PCR. Significantly increased expression levels were confirmed for 13 of these 15 transcripts, which suggest that these genes contribute to the salt tolerance of this plant. Among them, two transcription factors and several genes involved in isoprenoid biosynthesis were identified from mangrove trees as salt tolerance genes for the first time. The physiological significance of the increased expression of these genes in the long-term adaptation of mangrove trees to salt stress is discussed.

Answer 1

摘要
为了将抗盐压力基因从红树林植物中脱离出来，组建了一个红海榄根的互补DNA库，并用基于聚合酶链反应（PRC）的抑制消减杂交法（SSH）来帅选压力相关的基因。红树幼苗在两种条件下生长了8个月：一种是让其接触含盐量为3%的水，另一种是让其接触淡水。将接触含3%盐水的幼苗的互补DNA用作检验品，将接触淡水的幼芽用作驱动品。我们从SSH库中隔离出240个（DNA）并用正调节表现度序列标签作了标注。在这些正调节表达序列标签中，对48个独特克隆作了推定识别，并将其归入10个功能类别，如细胞救援与防护，次级代谢，蛋白质合成，新陈代谢等。从各类别选出15个基因，用实时逆转录酶-聚合酶链反应（RT-PCR）对其活跃度进行研究。记录结果确定这15个基因中的13个的活跃程度有了显著提高，这说明这些基因对此种植物的耐盐性起了作用。在它们中间，首次从红树植物中识别出了参与类异戊二烯生物合成的两个转录因子及多个基因具有耐盐性。论述了将红树植物长期接触盐压力的情况下这些基因活跃度提高在生理学上的意义。
【将就看吧...】

Answer 2

摘要：分离植物抗性基因从红树林植物，基因库的构建和筛选榄根压力有关的基因的聚合酶链反应（聚合酶链反应）的抑制性消减杂交（宋承宪）。红树林幼苗生长8个月的情况下：暴露于水与盐3%和接触到淡水。基因幼苗暴露于水和盐3%被用作测试仪和淡水萌发被用来作为一个司机。我们孤立和测序240个上调表达序列标签（无害环境技术）从消减文库。在这些上调无害，48独特的克隆公认的确定和划分为十个功能类，如细胞救援和国防，次生代谢，蛋白质合成，和代谢。十五个基因的不同类别选择和他们的表达研究了实时RT。显着增加的表达水平被确认为这13个15个记录，这表明，这些基因有助于这种植物的耐盐性。其中，转录因子和几个基因参与类异戊二烯生物合成被确定从红树耐盐基因为第一时间。生理意义，这些基因表达的增加在长期适应盐胁迫的红树林的讨论。
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Answer 3

隔离抗盐压力基因从红树植物、cDNA库的根构成和stylosa r与压力相关的基因筛查通过聚合酶链反应(PCR)——建立抑制的减杂交(SSH)。红树林幼苗生长为8个月在两个条件:暴露于含有3%的盐的水中和接触新鲜水。将暴露于含盐量为3%的盐水中的cDNA幼苗用作一个测试者并将cDNA淡水发芽设为一个驱动。我们分离并测序240上调表达序列标签(est)从SSH库。在这些est上调,48只独特的克隆被推定地识别和划分为10个功能类别,如细胞救援和国防、次生代谢、蛋白质合成,和新陈代谢。15个从不同类别的基因中选择出的基因与他们的表述正被进行实时逆转录聚合酶链反应研究。15个成绩中的13个被证实为水平显著增加,这表明这些基因导致这种植物耐盐。其中,两个转录因子和几个基因参与类异戊二烯合成从红树林被第一次确定为耐盐基因。对长期适应盐度的红树林基因的持续表述以及其生理意义正在成为讨论的话题。