Question

摘要翻译

人α-防御素HNP-2基因真核表达载体的构建
摘 要
目的：构建人α-防御素HNP-2真核表达载体。方法：利用PCR技术从质粒pET-28a（+）扩增人α-防御素HNP-2全长基因，HindⅢ和EcoRⅠ分别酶切目的基因和载体pcDNA3.1(+)，分别回收小片段和大片段，利用T4 DNA酶连接构建重组表达载体pcDNA3.1(+) /HNP-2，转化大肠杆菌DH5α，经快速PCR筛选和酶切、测序鉴定载体成功构建。 结果：经双酶切法酶切鉴定证实真核表达载体构建准确无误。结论：成功构建真核表达载体pcDNA3.1(+)/HNP-2，为进一步研究打下了基础

Answer 1

The construction of human's α-defensinHNP-2 gene eukaryotic expression vector.
Summary
Objective:Build human α-defensinHNP-2 eukaryotic expression vector.
Method:Amplify human's α-defensinHNP-2full-length gene, from plasmid pET-28a(+) by using PCR ,Hind Ⅲ andEcoR Ⅰenzyme cut target gene and vector pcDNA3.1(+),Separately recoveringsmallfragments andthe large fragment,UsingT4 DNA ligase to construct recombinant expression vector pcDNA3.1(+)/HNP-2 and escherichia coli DH5α,
Identify carrier successfully build by rapiding PCR screening and restriction enzyme digestion, sequencing.
Consequent :
Confirmed the eukaryotic expression vector construct accurate by double digestion method
digestion appraisal
Conclusion:
Constructed the eukaryotic expression vector pcDNA3.1(+)/HNP-2 successful.

Answer 2

你好，请问你是要翻译成什么？

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英文哈，谢谢