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Nowthatthegenome(DNA)ofhumansandmanyotherorganismshavebeensequenced,biologistsareturn... Now that the genome (DNA) of humans and many other organisms have been
sequenced, biologists are turning their attention to discovering how
the many thousands of structural and control genes -- the "worker
bees" of living cells that can turn genes on and off -- function.

To do that, they need to develop new techniques and tools. Scientists
in the Optical Microscopy group at the National High Magnetic Field
Laboratory at Florida State University, working in collaboration with
researchers from the University of Alberta in Canada and the
University of California, San Diego, have done just that, and in the
process have produced back-to-back articles in the prestigious journal
Nature Methods.

In the first paper, magnet-lab biologists Michael Davidson and Kristen
Hazelwood worked with researchers from the University of Alberta to
create two new fluorescent-protein biosensors, molecular "beacons"
that can tell if there is activity within a cell. The biosensors can
be used simultaneously to monitor two separate dynamic functions in a
single cell -- a key to understanding how different proteins and
enzymes (the biomolecules that cause chemical reactions) work together
to complete the daily chores that help cells grow and divide. Knowing
how cells work together can help researchers learn a great deal more
about tumors and developmental biology, among many other things.

The researchers improved a powerful technique used to monitor cellular
dynamics called fluorescence resonance energy transfer, or FRET. The
technique is used to examine a new class of biosensor molecules that
tether two fluorescent proteins together through an intervening
peptide (which is like a polymer). Several hundred of these new
biosensors have been developed over the past few years and are being
used by scientists around the world to study a variety of functions,
including programmed cell death, carbohydrate metabolism, cell
division, hormone stimulation, acidity changes -- just about any
cellular process that can occur.
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幽雪林月
2008-05-13 · TA获得超过970个赞
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现在的基因组图谱( DNA )的人类和许多其他的生物体已
测序,生物学家把注意力发现如何
许多数以千计的结构和控制基因-“工人
蜜蜂“的活体细胞可以转基因和关闭-功能。

要做到这一点,他们需要发展新技术和新工具。科学家
在光学显微镜研究小组在国家高磁场
实验室在佛罗里达州立大学,工作的协作与
研究人员从艾伯塔大学在加拿大和
美国加州大学,圣地牙哥,做了,只是,并在
过程中产生了背靠背的文章负盛名的杂志
性质的方法。

在第一份文件,磁体实验室的生物学家迈克尔戴维森和克里斯汀
hazelwood工作与研究人员从艾伯塔大学,以
创建两个新荧光蛋白的生物传感器,分子“信标”
它可以告诉,如果有活动的一间囚室。该生物传感器可以
被同时使用,以监察两个独立的动态功能,在一个
单细胞-的一个关键,以了解如何不同的蛋白质和
酶(生物分子造成的化学反应)携手合作
完成日常家务,帮助细胞生长和分化。明知
细胞如何携手合作,可以帮助研究人员了解了大量更多
关于肿瘤与发育生物学,其中包括许多其他事情。

研究人员改进了强大的技术用于监测细胞
所谓动态荧光共振能量转移,或烦恼。那个
技术是用来研究一类新的分子生物传感器
纽带二荧光蛋白一起通过一干预
多肽(即像一个聚合物) 。数百名,这些新
生物传感器已开发在过去几年中,并正
所使用的科学家,世界各地的研究多种功能,
包括程序性细胞死亡,碳水化合物代谢,细胞
司,激素刺激,酸度的变化-只要任何
细胞的过程中可能出现的。
xiaoyu701yyy
2008-05-14 · TA获得超过228个赞
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太长了,不好意思,能力有限哦!
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