Question

求助生物学论文摘要的英文翻译~~谢谢！拜托高手帮我翻译一下

目的：了解甲状腺激素受体β△基因在大鼠不同组织中的分布特点，熟悉用PT-PCR分析结果。方法：TRβ△基因在多种组织中均有表达，其中肝脏、脑、骨胳肌、心脏、肾脏表达最多。提取大鼠多处组织的mRNA, 以提取的mRNA为模板逆转录合成cDNA第一链。并以之为模板PCR扩增TRβ△双链cDNA。根据转录出的目的基因设计两条引物。采用荧光定量PCR的方法进行定量分析。结果：提取组织总RNA，OD260/OD280均介于1.7-2.0之间，OD260/230介于1.5-1.9之间。琼脂糖凝胶电泳鉴定PCR扩增结果显示,在预期的约160bp左右有目的扩增产物带。TRβΔ PCR产物的琼脂糖凝胶电泳鉴定结果表明，经PCR扩增出的DNA分子量大小为176bp的目的条带。 目的基因及管家基因定量测定，其扩增曲线和溶解曲线未见杂峰，同时融解曲主峰对应的Tm值与扩增产物的理论Tm相近。结论：研究结果显示TRβ△基因于mRNA水平的表达有一定的空间分布特点：肝，脑，脾，肾组织中均有该基因表达，且脾中的表达量明显高于其他组织。

Answer 1

Objective: To β △ thyroid hormone receptor gene in rats in the distribution of different characteristics, and is familiar with using PT-PCR analysis of the results. Method: TR β △ gene were expressed in a variety of organizations, including the liver, brain, skeletal muscle, heart, kidneys express the most. From various organizations in the mRNA, to extract the mRNA as a template retroviral cDNA synthesis of the first chain. And the template for PCR amplification TR β △ double-stranded cDNA. According to the purpose of gene transcription design of the two primers. Using fluorescence quantitative PCR method of quantitative analysis. Results: The Organization of extracting RNA, OD260/OD280 are between 1.7-2.0 between, OD260/230 range between 1.5-1.9. Identification of agarose gel electrophoresis of PCR results showed that in the expected around about 160 bp PCR products with a purpose. TR βΔ PCR product of the identification of agarose gel electrophoresis results showed that the PCR amplification of DNA molecular size to 176 bp for the purpose of the zone. Manager gene and gene quantitative determination of its amplification curve and no dissolution of miscellaneous peak, while the corresponding peak melting song Tm value and amplified products similar to the theory of Tm. Conclusion: The results showed that TR β △ gene expression at the mRNA level of a certain spatial distribution of characteristics: the liver, brain, spleen, kidney tissue in both the gene expression, the expression and spleen was significantly higher than that of other organizations.

Answer 2

Goal: Understood the thyroxine acceptor β△ gene in big mouse different organization distributed characteristic, is familiar with with PT-PCR analyzes the result. Method: TRβ△ the gene has the expression in many kinds of organizations, in which liver, the brain, the skeleton muscle, the heart, the kidney expression are most. Withdraws big mouse many place organizations mRNA, withdraws mRNA is the template counter duplication synthesizes the cDNA first chain. And expands take it as template PCR increases the goal gene which TRβ△ the double strand cDNA. basis copies to design two to direct the thing. Uses fluorescence quota PCR the method to carry on the quantitative analysis. Finally: The extraction organizes total RNA, OD260/OD280 to be situated between between 1.7-2.0, OD260/230 is situated between between 1.5-1.9. The agarose gel electrophoresis appraises PCR to expand increases the result to demonstrate that, has the goal about anticipated 160bp to expand approximately increases the product belt .TRβΔ PCR product the agarose gel electrophoresis appraisal result to indicate, expands the DNA molecular weight size after PCR which increases is the 176bp goal banding. The goal gene and the steward gene quantitative determination, it expands increases the curve and the curve of solubility has not seen the mixed peak, simultaneously melts the tune prominent peak correspondence Tm value with to expand increases the product theory Tm to be close. Conclusion: The findings showed TRβ△ the gene has certain spatial distribution characteristic in the mRNA level expression: The liver, the brain, the spleen, in the kidney organization has this gene expression, also in the spleen expression quantity is higher than other organizations obviously.