急！高分追加！帮忙做几道分子生物学的题
HepatitisBviruscancauselivercancerandgarlicisbelievedtohaveanti-viraleffectinmanycoun...
Hepatitis B virus can cause liver cancer
and garlic is believed to have anti-viral effect in many countries. You are
hired by a pharmaceutical company to invest the effect of garlic extraction on gene
expression in cells isolated from viral induced liver cancers. Draw
a flow chart (use arrows to link steps you will take) to study the gene
expression changes in these cells with brief explanation.
The
Chinese herb star anise is used to extract a compound for making an antiviral
drug, Tamiflu. To study the potential side effects, you are leading a team to
investigate whether Tamiflu can make gene expression changes in viral infected
and normal human cells. Draw a flow chart (use arrows to link steps you will
take) to study the gene expression changes in these cells with brief
explanation.
You have joined a research laboratory
to assess the mutagenicity of a new drug. Suggest a procedure to do so and
explain to the team leader the principle of this assay.
As a scientist at a environment
protection agency in a small town, you are investigating a compound released by
a company to local water ways for its mutagenicity. Describe a possible assay
to determine mutagenicity and explain its principle.
英文或者中文回答都可以。追加多少分都行 展开
and garlic is believed to have anti-viral effect in many countries. You are
hired by a pharmaceutical company to invest the effect of garlic extraction on gene
expression in cells isolated from viral induced liver cancers. Draw
a flow chart (use arrows to link steps you will take) to study the gene
expression changes in these cells with brief explanation.
The
Chinese herb star anise is used to extract a compound for making an antiviral
drug, Tamiflu. To study the potential side effects, you are leading a team to
investigate whether Tamiflu can make gene expression changes in viral infected
and normal human cells. Draw a flow chart (use arrows to link steps you will
take) to study the gene expression changes in these cells with brief
explanation.
You have joined a research laboratory
to assess the mutagenicity of a new drug. Suggest a procedure to do so and
explain to the team leader the principle of this assay.
As a scientist at a environment
protection agency in a small town, you are investigating a compound released by
a company to local water ways for its mutagenicity. Describe a possible assay
to determine mutagenicity and explain its principle.
英文或者中文回答都可以。追加多少分都行 展开
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抱歉，虽然能回答，但是具体方案作出来要耗掉我整个下午，做HIGH了甚至熬夜都做不完^^
简单陈述下，如果我来做这些flow chart的话，每个都包括三个大的部分：
1,模型选取，以第一题为例，是cells isolated from viral induced liver cancers，这里就涉及由于乙肝导致肝癌的病人标本获取（对照组是无肝脏疾病的人），以及从这些标本中分离培养原代细胞。
2.干预手段，以第一题为例，是加入garlic extraction，这里推荐在处理时间和处理量上建立梯度（空白组是只加用于提取garlic extraction的提取液）。
3.检测指标，以第一题为例，on gene expression in cells，这里您得仔细选取去检测哪些基因了，因为讨论的癌症，套路一般是增值、凋亡、迁移，那就选取和增值、凋亡、细胞粘附相关的基因，然后抽rna做RT-PCR，抽蛋白做western。
第二题类似，建模和干预已经在题目里明确了。
第三题就很开放了，我写的话会直接上小鼠，注射小鼠，检测成年鼠和胎鼠，除了肉眼观察有没有畸形外，去五脏六腑肌肉神经做切片并染色，再过分点取血取尿验一系列生化指标，交配个几代看生殖能力，再过分点做点行为学实验看有没有精神病。
第四题和第三题类似，都是看有没有mutagenicity，于是除了对耗子注射物不一样，其他都可以类似。
胡言乱语这么多，希望对您有所帮助~
简单陈述下，如果我来做这些flow chart的话，每个都包括三个大的部分：
1,模型选取，以第一题为例，是cells isolated from viral induced liver cancers，这里就涉及由于乙肝导致肝癌的病人标本获取（对照组是无肝脏疾病的人），以及从这些标本中分离培养原代细胞。
2.干预手段，以第一题为例，是加入garlic extraction，这里推荐在处理时间和处理量上建立梯度（空白组是只加用于提取garlic extraction的提取液）。
3.检测指标，以第一题为例，on gene expression in cells，这里您得仔细选取去检测哪些基因了，因为讨论的癌症，套路一般是增值、凋亡、迁移，那就选取和增值、凋亡、细胞粘附相关的基因，然后抽rna做RT-PCR，抽蛋白做western。
第二题类似，建模和干预已经在题目里明确了。
第三题就很开放了，我写的话会直接上小鼠，注射小鼠，检测成年鼠和胎鼠，除了肉眼观察有没有畸形外，去五脏六腑肌肉神经做切片并染色，再过分点取血取尿验一系列生化指标，交配个几代看生殖能力，再过分点做点行为学实验看有没有精神病。
第四题和第三题类似，都是看有没有mutagenicity，于是除了对耗子注射物不一样，其他都可以类似。
胡言乱语这么多，希望对您有所帮助~
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