Question

看不懂，有人帮我翻译下吗？谢谢

Cell-substrate adhesion was treasured by plating cells preincu-bated for 24 h with or without carotenoids in 96-well plates (10,000 cells/well), incubating for 10 min before inverting and washing(4-times with RPMI), and enumeration of attached cells by CYQuant.PMA (120 nmol/L) addition served as a positive control, and back ground adhesion (0-2.0% in all groups) was determined by immediate washing
Statistical analysis. Data were analyzed by 1-way ANOVA todetermine whether there were any significant differences among the groups in each experiment, followed by the Tukey-Kramer pairwise multiple comparison test to determine the significance of differences among groups. Data are expressed as means士SEM from multiple independent experiments, each performed in triplicate or quadruplicate wells. Differences were considered significant at P<0.05.
RESULTS
Cellular enrichment with carotenoids. In vitro preenrich-ment of human serum with a specific carotenoid effectively increased serum concentration of that carotenoid (Table 1).Attempts to achieve greater enrichments by the addition of a higher stock concentration yielded large variations in serum carotenoid concentrations achieved after incubation and filtration (data not shown). Given the greater consistency in TABLE1
media enrichment using 1 tnmol/L stock solutions, all cell enrichments were done in this manner. Incubation of U937 with media containing the preenriched serum for 24 h resulted in cellular enrichment (Table 2). No further cellular enrich-ment with carotenoids was achieved after 24 h (data not shown). Cell viability, assessed both pre- and postexposure to
carotenoids, was >= 90%.

Answer 1

细胞-基质的粘附珍惜镀细胞preincu - bated 24 h后有或没有的类胡萝卜素在96孔板（ 10000细胞/好） ，孵化为10分钟前，逆变和洗衣机（ 4倍，与rpmi ） ，以及枚举重视细胞cyquant.pma （ 120 nmol / L ）的服务，此外作为一个积极的控制，回到地面粘附（ 0-2.0 ％ ，在所有群体）是取决于即时洗
统计分析。数据分析由一程的ANOVA todetermine是否有任何显着性差异各组在每个实验，其次是tukey -克莱默成对多重比较测试，以判断的意义之间的分歧团体。数据表示为手段，士教统局局长由多个独立的实验，每个表现在三个或quadruplicate水井。分歧被认为是显着的在P < 0.05 。
结果
细胞富集的类胡萝卜素。在体外preenrich - ment人血清与特定的类胡萝卜素有效地提高了血药浓度认为，类胡萝卜素（见表1 ） 。试图实现更大的强化，通过增加高浓度的股票取得了大的变化血清中类胡萝卜素的浓度达到后，孵化和过滤（数据未显示） 。鉴于更大的一致性，在表
媒体富集使用一tnmol /升股市的解决方案，所有细胞的强化做了以这种方式。孵化的U937与媒体载preenriched血清24 h后，导致细胞的富集（见表2 ） 。没有进一步的蜂窝丰富- ment与类胡萝卜素达到24 h后（数据未显示） 。细胞的可行性，评估前和postexposure ，以
类胡萝卜素，是> = 90 ％ 。