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摘要:人参皂苷Re是人参中提取的一种皂苷成分单体,也是人参皂苷发挥药理作用最重要活性成分之一,本试验采用大孔树脂法测定人参中人参皂苷Re的成分,将人参须根用70%乙醇超声... 摘要:人参皂苷Re是人参中提取的一种皂苷成分单体,也是人参皂苷发挥药理作用最重要活性成分之一,本试验采用大孔树脂法测定人参中人参皂苷Re的成分,将人参须根用70%乙醇超声提取三次并合并浓缩,用NKA12大孔树脂柱分离,流动相分别用水、30%乙醇、95%乙醇,将30%乙醇所得组分浓缩,用甲醇与水反复重结晶得人参皂苷Re无色针晶。HPLC法得人参皂苷Re谱图,面积归一法测定纯度为98.54%。结论:该方法稳定、易操作、纯度较高、可反复操作。
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TiTiweb3社交
2008-06-18 · TA获得超过2878个赞
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Abstract: ginsenoside Re ginseng extract is a saponin component monomer, but also play a ginsenoside pharmacological action of one of the most important active ingredient, the pilot used the resin-Determination of ginseng in the composition of ginsenoside Re, Ginseng fibrous roots will use 70 percent ethanol and ultrasound from the merger three times concentrated, with NKA12 macroporous resin column separation, the mobile phase were water, 30 percent ethanol, 95 percent ethanol, will be 30 percent ethanol concentration from the components used Methanol and water was repeatedly re-crystallization of ginsenoside Re-colorless crystal. By HPLC in the ginsenoside Re spectrum, the area under an Act of purity is 98.54 percent. Conclusion: This method is stable and easy to operate, high purity, can be repeated operations.
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悲剧520
2008-06-18
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Ginsenoside Re ginseng extract is a saponin component monomer, is also ginsenoside play pharmacological effect one of the most important active ingredient, the macroporous this experiment using resin Determination of ginseng in the composition of ginsenoside Re, ginseng Fibrous roots with 70 percent ethanol and ultrasound from the merger three times concentrated, with NKA12 macroporous resin column separation, the mobile phase were water, 30 percent ethanol, 95 percent ethanol, will be 30 percent ethanol from enrichment components, methanol And repeatedly re-crystallization of water in the ginsenoside Re-colorless crystal. HPLC method in the ginsenoside Re spectrum
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