翻译成英文,谢谢
1.关于营养缺陷型菌株生产赖氨酸的问题。在许多微生物中,可用天冬氨酸作原料,通过分支代谢途径合成出赖氨酸、苏氨酸和甲硫氨酸。但在代谢过程中,一方面由于赖氨酸对天冬氨酸激酶...
1.关于营养缺陷型菌株生产赖氨酸的问题。
在许多微生物中,可用天冬氨酸作原料,通过分支代谢途径合成出赖氨酸、苏氨酸和甲硫氨酸。但在代谢过程中,一方面由于赖氨酸对天冬氨酸激酶有反馈抑制作用,另一方面,由于天冬氨酸除用于合成赖氨酸外,还要作为合成甲硫氨酸和苏氨酸的原料,因此,在正常细胞内,就很难累积较高浓度的赖氨酸。工业上利用谷氨酸棒杆菌的高丝氨酸缺陷型菌株作为赖氨酸的发酵菌种。由于它不能合成苏氨酸脱氢酶,故不能合成高丝氨酸,也不能产生苏氨酸和甲硫氨酸,在补以适量高丝氨酸的条件下,可在较高糖浓度和铵盐的培养基上,产生大量的赖氨酸。
2.传统经典的诱变生产(赖氨酸)技术和分子克隆技术之优缺点比较
赖氨酸的高产菌株已通过经典的诱变法获得,但要再提高这些诱变株的产量就很困难。
而分子克隆技术生产,产量大,节约成本。但也会带来一些问题。 展开
在许多微生物中,可用天冬氨酸作原料,通过分支代谢途径合成出赖氨酸、苏氨酸和甲硫氨酸。但在代谢过程中,一方面由于赖氨酸对天冬氨酸激酶有反馈抑制作用,另一方面,由于天冬氨酸除用于合成赖氨酸外,还要作为合成甲硫氨酸和苏氨酸的原料,因此,在正常细胞内,就很难累积较高浓度的赖氨酸。工业上利用谷氨酸棒杆菌的高丝氨酸缺陷型菌株作为赖氨酸的发酵菌种。由于它不能合成苏氨酸脱氢酶,故不能合成高丝氨酸,也不能产生苏氨酸和甲硫氨酸,在补以适量高丝氨酸的条件下,可在较高糖浓度和铵盐的培养基上,产生大量的赖氨酸。
2.传统经典的诱变生产(赖氨酸)技术和分子克隆技术之优缺点比较
赖氨酸的高产菌株已通过经典的诱变法获得,但要再提高这些诱变株的产量就很困难。
而分子克隆技术生产,产量大,节约成本。但也会带来一些问题。 展开
6个回答
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1. Nutrient-deficient strain on the production of lysine.
In many microbes, the acid can be used as raw materials, through the branches of the synthetic pathway of lysine, threonine and methionine. But in the process of metabolism, on the one hand, as a result of acid lysine feedback inhibition of kinase, on the other hand, due to not only be used for synthesis of acid lysine, but also as a synthesis of methionine and Thr of raw materials, therefore, in normal cells, it is hard to accumulate high concentrations of lysine. Industrial use of Corynebacterium glutamicum high-serine as a lysine-deficient strain of the bacteria fermentation. Because it can not be synthesized threonine dehydrogenase, it can not synthesis Serine high, we can not produce methionine and threonine, serine up to the high amount of conditions, can be high in sugar concentration of salts and culture On the base, a large amount of lysine.
2. Traditional production of the classic mutation (lysine) technology and molecular cloning of comparative advantages and disadvantages
High-yielding strains of lysine has been through the classic method to obtain mutation, but they have to increase the mutation of the production line is very difficult.
Molecular cloning and production, production, cost-effective. But it can also cause some problems.
保证对!
In many microbes, the acid can be used as raw materials, through the branches of the synthetic pathway of lysine, threonine and methionine. But in the process of metabolism, on the one hand, as a result of acid lysine feedback inhibition of kinase, on the other hand, due to not only be used for synthesis of acid lysine, but also as a synthesis of methionine and Thr of raw materials, therefore, in normal cells, it is hard to accumulate high concentrations of lysine. Industrial use of Corynebacterium glutamicum high-serine as a lysine-deficient strain of the bacteria fermentation. Because it can not be synthesized threonine dehydrogenase, it can not synthesis Serine high, we can not produce methionine and threonine, serine up to the high amount of conditions, can be high in sugar concentration of salts and culture On the base, a large amount of lysine.
2. Traditional production of the classic mutation (lysine) technology and molecular cloning of comparative advantages and disadvantages
High-yielding strains of lysine has been through the classic method to obtain mutation, but they have to increase the mutation of the production line is very difficult.
Molecular cloning and production, production, cost-effective. But it can also cause some problems.
保证对!
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1. about nutrition flaw strain production lysine question.
In many microorganisms, the available aspartic acid makes raw material, synthesizes the lysine, the threonine and the methionine through the branch pathways.But in metabolism process, on the one hand because the lysine has the feedback inhibition function to the aspartic acid activating enzyme, on the other hand, because the aspartic acid besides uses in synthesizing the lysine, but also must take the synthesis methionine and threonine raw material
Therefore, in the normal cell, very is difficult to accumulate the high density the lysine.In the industry takes the lysine using the glutanic acid good bacillus high serine flaw strain the fermentation mold mushroom spawn.Because it cannot synthesize the threonine dehydrogenase, therefore cannot synthesize the high serine, also cannot produce the threonine and the methionine, in makes up by under the right amount high serine condition, may in the high sugar density and on the ammonium salt culture medium, produces the massive lysine
2. traditional classics mutafacient production of (lysine) good and bad points of comparison lysine technical and the molecular clone
technology high production strain has obtained through the classics mutafacient law, but must again enhance these mutafacient output very to be difficult.
But the molecular clone technology production, the output is big, saves the cost.But also can bring some questions
In many microorganisms, the available aspartic acid makes raw material, synthesizes the lysine, the threonine and the methionine through the branch pathways.But in metabolism process, on the one hand because the lysine has the feedback inhibition function to the aspartic acid activating enzyme, on the other hand, because the aspartic acid besides uses in synthesizing the lysine, but also must take the synthesis methionine and threonine raw material
Therefore, in the normal cell, very is difficult to accumulate the high density the lysine.In the industry takes the lysine using the glutanic acid good bacillus high serine flaw strain the fermentation mold mushroom spawn.Because it cannot synthesize the threonine dehydrogenase, therefore cannot synthesize the high serine, also cannot produce the threonine and the methionine, in makes up by under the right amount high serine condition, may in the high sugar density and on the ammonium salt culture medium, produces the massive lysine
2. traditional classics mutafacient production of (lysine) good and bad points of comparison lysine technical and the molecular clone
technology high production strain has obtained through the classics mutafacient law, but must again enhance these mutafacient output very to be difficult.
But the molecular clone technology production, the output is big, saves the cost.But also can bring some questions
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建议需要专业英语翻译最好去对应区域发帖子
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难度太高了吧,才20分……建议你去谷歌在线翻译吧
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哦~专业
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我知道大家每天都在使用手机。但是有谁知道手机最常用而且也是最重要的功能--拨打、接听无线电话和发送、接受短信的发展由来呢?
mobile
phones
are
widely
used
every
day
in
this
world.
many
people
know
how
to
make
phone
calls,
send
and
receive
short
messages
via
,
which
are
the
most
basic
and
important
function
of
mobile
phones,
but
only
few
really
knows
their
history
of
development.
我们现在使用的手机都是2G和3G的手机,而第一代的手机(1G)是在1973年,Motorola公司所发明的。当时的1G手机最大的成就是去掉了将电话线连接到网络的用户线,用户可以在任何地方无线接受和拨打电话。不过当时的手机只能用于拨打和接听无线电话,但是在
1992年,世界上第一条短信在英国发送成功。但是知道2G手机的出现,短信才在1999年在世界各国开始流行。
mobile
phones
we
are
using
today
are
mainly
the
second
and
third
generation.
the
first
generation
of
mobile
phones
were
invented
by
motorola
in
1973.
at
that
time,
the
biggest
selling
point
was
that
users
can
make
and
receive
phone
calls
wireless
anywhere.
but
short
message
service
was
not
available
then.
in
1992,the
first
short
message
was
sent
successfully
in
britain.
the
service
was
not
popular
until
the
second
generation
of
mobile
phones
appeared
in
1999.
mobile
phones
are
widely
used
every
day
in
this
world.
many
people
know
how
to
make
phone
calls,
send
and
receive
short
messages
via
,
which
are
the
most
basic
and
important
function
of
mobile
phones,
but
only
few
really
knows
their
history
of
development.
我们现在使用的手机都是2G和3G的手机,而第一代的手机(1G)是在1973年,Motorola公司所发明的。当时的1G手机最大的成就是去掉了将电话线连接到网络的用户线,用户可以在任何地方无线接受和拨打电话。不过当时的手机只能用于拨打和接听无线电话,但是在
1992年,世界上第一条短信在英国发送成功。但是知道2G手机的出现,短信才在1999年在世界各国开始流行。
mobile
phones
we
are
using
today
are
mainly
the
second
and
third
generation.
the
first
generation
of
mobile
phones
were
invented
by
motorola
in
1973.
at
that
time,
the
biggest
selling
point
was
that
users
can
make
and
receive
phone
calls
wireless
anywhere.
but
short
message
service
was
not
available
then.
in
1992,the
first
short
message
was
sent
successfully
in
britain.
the
service
was
not
popular
until
the
second
generation
of
mobile
phones
appeared
in
1999.
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