Question

求汉译英，高分。在线等。机翻绕道！专业名词可以不翻译

2 首次建立了鸭LRRC3B、L1CAM基因的实时荧光定量PCR方法
根据本实验室对鸭肝脏转录组高通量测序获得的鸭LRRC3B基因、鸭L1CAM基因序列，建立了检测鸭LRRC3B、L1CAM基因的实时荧光定量PCR方法。其中鸭LRRC3B基因的实时荧光定量PCR方法的扩增效率为100.32%，相关系数为0.9990，线性范围是4.66×104～4.66×109拷贝/μL，最低检测限为4.66×103拷贝/μL，熔解曲线分析为单特异峰，无非特异性扩增和引物二聚体，批内变异系数为1.59%～2.04%，批间变异系数为1.00%～2.22%。鸭L1CAM基因的实时荧光定量PCR方法的扩增效率为103%，相关系数为0.9990，线性范围是2.17×103～2.17×107拷贝/μL，最低检测限为2.17×103拷贝/μl，熔解曲线分析为单特异峰，无非特异性扩增和引物二聚体，批内变异系数为 1.50%～2.61%，批间变异系数为1.36%～3.03% 。
这两个基因的实时荧光定量PCR方法的建立为对鸭LRRC3B、L1CAM基因功能的研究以及这两个基因在病毒感染过程的转录情况的研究提供了有效的方法。

Answer 1

人工翻译，请审阅
2 For the first time a real time FQ-PCR method is established for duck LRRC3B and duck L1CAM genes
According to the sequences of duck LRRC3B and duck L1CAM gene obtained by the present laboratory using high-throughtput sequencing of duck liver, a real time FQ-PCR method for detecting duck LRRC3B and duck L1CAM genes is established. Wherein the amplification efficiency of the real time FQ-PCR method for duck LRRC3B gene is 100.32%, with a correlation coefficient of 0.9990, a linear range of 4.66 × 104 ~ 4.66 × 109 copies / μL, the lowest detection limit of 4.66 × 103 copies / μL, melting curve analysis having a single specific peak, no nonspecific amplification and primer dimers, and an intra-assay variation of 1.59 % ~ 2.04%, an inter-assay variation of 1.00% ~ 2.22%. The amplification efficiency of the real time FQ-PCR method for duck L1CAM gene is 103%, with a correlation coefficient of 0.9990, a linear range of 2.17 × 103 ~ 2.13× 107 copies / μL, the lowest detection limit of 2.17× 103 copies / μL, melting curve analysis having a single specific peak, no nonspecific amplification and primer dimers, and an intra-assay variation of 1.50% ~ 2.61%, an inter-assay variation of 1.36% ~ 3.03%.
The establishement of the real-time F!-PCR method for these two genes provides an efficient approach for the research on the functions of duck LRRC3B and L1CAM genes and the research on the transcription of these two genes in the case of viral infection.