Question

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1. 4 14CO2-feeding to leaf and fruit of satsuma mandarin
14 CO2-feeding experiments were conducted as described by CHEN et al [6 ] . At each of the four developmental stages (27 July , 18 September , 11 October , and2 November) , a fruit , or a leaf closest to an attached fruit , was selected and enclosed in polythene bags in which 1. 85 ×105 Bq of 14CO2 were released at 10 :00 -12 :00 in a sunshine day. The stem was ringed below the fed leaves or fruits to prevent basipetal transport before feeding. Fruits were harvested at 6 , 23 and 47h after feeding 14CO2 for 1h , then separated into peel , vascular bundle , segment epidermis and juice sacs. These components were frozen in liquid nitrogen and then boiled in 80 % ethanol . Sample radioactivity was determined by Wallac 1410 liquid scintillation counter (Pharmacia Company , USA) .

Answer 1

1. 414co2喂养的叶子和果实町国语 14二氧化碳喂养实验进行了描述陈观Al6. 每四个发展阶段(1918年7月27日September,11日,11月测量)、果实、叶子最接近的附水果、选择和聚乙烯密封袋,1. 乘105路,8514co2获释10:00-12:00有阳光的日子. 以下是被灌成的叶子或水果,以防止在运输basipetal喂养. 水果收获6、23、47h后喂养14co2为h,则分为去皮、血管束,部分表皮和汁囊. 这些组件都在液态氮中冷冻,然后用80%的乙醇. 样品是由放射性液体闪烁Wallac1410柜台(Pharmacia公司,USA)