Question

请高手帮我翻译下面的文章。。。不要网上在线翻译的（不给分），要高手亲自翻译的，翻译得好另外加高分(6)

The Mirror Orientation Selection (MOS) method has been recently developed to eliminate type II background from SSH-generated libraries (Rebrikov et al., 2000).

Subtraction of bacterial genomes
SSH method has been adapted for comparison of bacterial genomes (Akopyants et al., 1998). Genes that are present in certain isolates of a given bacterial species and absent or substantially different in others can be of great interest biologically. Some may determine strain-specific traits such as drug resistance, bacterial surface structure, or restriction-modification. Of special importance in infectious disease are the "pathogenicity islands" (PAIs), multigene segments of virulent strains that tend to be absent from avirulent members of the same species and help determine the nature and severity of disease.

To select strain-specific genes in bacterial genomes using SSH, pools of digested genomic DNA from the strain of interest (tester) are deprived of sequences also present in the reference strain (driver) by judicious use of hybridization and PCR. The remaining tester-enriched DNA fragments are then cloned for further analysis.

Answer 1

镜子取向选择(MOS)方法最近被开发从SSH引起的图书馆(等Rebrikov消灭类型II背景， 2000)。
细菌染色体SSH方法的减法为细菌染色体适应了(等Akopyants比较， 1998)。 是存在一个特定细菌种类某些孤立和缺席或者极大地不同的在其他的基因可以是巨大利益生物。 一些也许确定张力具体特征例如药物抵抗、细菌表面结构或者制约修改。 特别重要在传染病是“致病性海岛” (PAIs)，倾向于是缺席同一个种类的无毒成员和帮助确定疾病本质和严肃剧毒张力的multigene段。 使用SSH要选择张力具体基因在细菌染色体，被消化的genomic脱氧核糖核酸水池从张力利益(测试器)也被剥夺序列当前在参考张力(司机)由对杂交和PCR的明智的用途。 剩余的测试器被丰富的脱氧核糖核酸片段为进一步分析然后被克隆。