各位大侠 帮帮忙，翻译一下这段话（英文翻译） 10
Abstract：Theinteractionbetweenunsaturatedphosphatidylcholinesandeitherexogenousorendo...
Abstract：The interaction between unsaturated phosphatidylcholines and either exogenous or endogenous (produced by the enzyme system involving myeloperoxidase (MPO), H2O2, and Cl–) hypochlorite was studied in multilayer liposomes containing oleic, linoleic, and arachidonic acid residues using MALDI TOF mass spectrometry. At pH 7.4, hypochlorite reacts with the dou ble bond of the oleic acid residue in 1 -stearoyl -2 -oleoyl -sn -glycero -3- phosphocholine producing oleic acid chlorohydrin as the main product. Minor amounts of glycols and epoxides were also detected. The main products of the reaction of hypochlorite with 1 -stearoyl -2- linoleyl -sn -glycero -3 -phosphocholine were mono- and di- chlorohydrins of linoleic acid. The signals of monoglycol, epoxide, and glycol or epoxide containing monochlorohydrin derivatives were also present in the mass spectrum. The main products of the reaction of hypochlorite with 1 -stearoyl -2 -arachidonyl-sn-glycero-3 -phosphocholine were lysophosphatidylcholine (1 –stearoyl- sn -glycero -3 -phosphocholine) and mono , di , and trichlorohydrin. Monoglycol and its derivatives containing one or two chlorohydrin groups were also detected. Along with those, carbonyl compounds (aldehyde and acid) formed as a result of double bond breakage in fifth position of arachidonate weredetected. Monochlorohydrin was also found when liposomes comprising
1-stearoyl -2-oleoyl -sn-glycero-3-phosphocholine were incubated in the presence of enzymatic mixture, MPO + H2O2 + Cl–, at pH 6.0. In the absence of the enzyme or either of its substrates (H2O2 or Cl–) or in the presence of the MPO inhibitor (sodium azide) or hypochlorite scavengers (taurine or methionine), monochlorohydrin formation was not observed. These data confirm the suggestion that just the hypochlorite generated in MPO -catalysis provides for chlorohydrin formation. Thus, the use of MALDI -TOF mass spectrometry has shown, along with chlorohydrins, glycols and epoxides as the products of hypochlorite interaction with unsaturated phosphatidylcholines at physiological pH. It was first determined that hypochlorite breaks double bonds in polyunsaturated phosphatidylcholine and also causes lysophosphatidylcholine formation. 展开
1-stearoyl -2-oleoyl -sn-glycero-3-phosphocholine were incubated in the presence of enzymatic mixture, MPO + H2O2 + Cl–, at pH 6.0. In the absence of the enzyme or either of its substrates (H2O2 or Cl–) or in the presence of the MPO inhibitor (sodium azide) or hypochlorite scavengers (taurine or methionine), monochlorohydrin formation was not observed. These data confirm the suggestion that just the hypochlorite generated in MPO -catalysis provides for chlorohydrin formation. Thus, the use of MALDI -TOF mass spectrometry has shown, along with chlorohydrins, glycols and epoxides as the products of hypochlorite interaction with unsaturated phosphatidylcholines at physiological pH. It was first determined that hypochlorite breaks double bonds in polyunsaturated phosphatidylcholine and also causes lysophosphatidylcholine formation. 展开
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