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[摘要]:目的:利用电纺法,制备大肠杆菌噬菌体为模版的分子印迹聚合膜。要求在本次试验中熟悉科研的整个过程,包括资料的收集整理、课题设计、实验操作和专业论文的撰写。同时提高... [摘要]:目的:利用电纺法,制备大肠杆菌噬菌体为模版的分子印迹聚合膜。要求在本次试验中熟悉科研的整个过程,包括资料的收集整理、课题设计、实验操作和专业论文的撰写。同时提高学生的科研思维能力和实际动手能力。
方法:应用PEG沉淀+氯仿抽提法浓缩提纯噬菌体达到1012/ml后,通过调节PVA的浓度、流量、电压、高度等条件进行纺丝,制备成膜后通过扫描电镜(SEM)观察纺丝溶液的浓度、流量、电压、高度等条件对纺丝纤维的形貌和直径的影响。利用静电纺丝技术在以上摸索出的条件下制备以PVA为“壳”、大肠杆菌噬菌体为“芯”的分子印迹膜,并通过双层琼脂观察噬菌斑的方法检测封装后噬菌体的生物活性。
结果:成功浓缩提纯噬菌体达到1012/ml以上。在条件摸索过程中制备以水为溶剂的PVA浓度为7%(w/w)的膜2张;在加入乙醇和SM后,纺丝很不稳定,出现大量断丝、串珠、滴液现象,无法成膜,由于时间原因,未继续进行,实验由此终止。转而进行PVA纺丝纤维的形貌、直径等与PVA浓度、电压间的关系摸索
结论:①由所制备的几张膜的观察结果表明,随着纺丝溶液浓度的增加,纤维直径逐渐增大,纤维直径的分布也随之增大;随着电压的增大,纤维直径没有明显变化。
②实验准备不足,未对噬菌体与纺丝聚合物基质PVA的相容性进行评估。
[关键词]:噬菌体 效价 静电纺丝 PVA
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2009-05-28 · 超过17用户采纳过TA的回答
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Objective: to use electricity spinning method for preparation of e. coli phage, molecularly imprinted polymer membranes of the template. In this test requirements with the whole process of scientific research, including data collection, the subject designing, experimental operation and professional writing the thesis. While improving students' scientific thinking ability and practical ability.
Methods: using mpeg precipitation + chloroform extraction smoke concentration purification phage reach 1012 / ml, concentration of PVA by adjusting voltage, high flow rate, and spinning, the preparation conditions after film by scanning electron microscope (SEM) spinning solution concentration, flow, voltage, high on spinning fiber diameter and the morphology of the influence. Using the electrostatic spinning technology in preparation for the conditions for the "shell" with PVA, escherichia coli phage as "core" of molecularly imprinted membrane, and through the double plaque method of observing AGAR bite after encapsulation testing of bioactive phage.
Results: the successful condensed phage reach 1012 / ml. In the process of preparation conditions with water groping for solvent for 7% PVA concentration (w/w) of membrane 2, Before joining ethanol and SM, spinning is unstable, lots of broken wire and beads, liquid membrane, not because of time, not continue, experiment which terminates. Turn on the morphology, PVA spinning fiber diameter with PVA concentration, the relationship between voltage
Conclusions: (1) by the preparation of several membranes observed results show that, with the increase of spinning solution concentration, fiber diameter increase gradually, the distribution of fiber diameter with, With the increase of fiber diameter, voltage did not change significantly.
2, the experiment of polymer matrix phage and spinning compatibility of PVA were evaluated.
[key] : a phage maximizing electrostatic spinning PVA
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[Abstract]: Objective: To make use of electric spinning method for preparation of E. coli phage template molecular imprinting polymer film. Requirements in this experiment are familiar with the whole process of scientific research, including data collection, the subject design, the experimental operation and the writing of professional papers. At the same time improve the thinking ability of students to scientific research and practical hands-capacity.
Methods: PEG precipitation + chloroform extraction purification of phage enrichment after reaching 1012/ml, by adjusting the concentration of PVA, flow, voltage, and other conditions of the high spinning, film prepared by scanning electron microscopy (SEM) observation of spin silk solution concentration, flow rate, voltage, and other high fiber spinning conditions on morphology and diameter of the impact. Electrospinning technology use in the above conditions and explore the preparation of a PVA for the "shell", E. coli phage as a "core" of the molecularly imprinted membrane, and through observation of double-layer agar method Plaque packaged phage detection of biological activity.
Results: The success of purification of phage concentration to achieve the above 1012/ml. Groping in the process of the preparation conditions with water as the solvent for the PVA concentration of 7% (w / w) of the membrane 2; in the SM by adding ethanol and, after spinning a very unstable and a large number of broken wires, beads, drip phenomenon, not film, due to time reasons, did not continue, the resulting termination of the experiment. Turn to the morphology of PVA fiber spinning, the diameter, such as PVA concentration, exploring the relationship between voltage
CONCLUSION: ① prepared by the observation of a few films The results showed that the concentration of spinning solution with the increase in fiber diameter increases, the distribution of fiber diameter increases; as the voltage increases, fiber diameter did not change significantly.
② experiment to make adequate preparations, phage with no spinning PVA polymer matrix to assess the compatibility.
[Key words]: phage titer PVA electrospinning
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