Question

这句话怎么翻译？

Comparison of restriction map of the CK gene with that of
subtilisin Carlsberg and its isoforms. For the differentiation of
CK and subtilisin Carlsberg, PCR was performed five times to
eliminate Taq DNA polymerase error. Each PCR product was
inserted into pGEM-7Zf(1) vector and transformed to E. coli
JM109. Each plasmid containing the PCR product of the CK
gene was digested with several restriction enzymes, and the
same results were obtained from all of the restriction maps. A
comparison with the restriction enzyme maps of subtilisin
Carlsberg and its isoforms, shown in Fig. 4, indicates that the
restriction enzyme sites of the CK gene are different from that
of Bacillus licheniformis NCIB 6816 (subtilisin Carlsberg) (9) at
the PstI, AccI, and HindII sites. Differences are also evident
with B. licheniformis 11594 (20) at the NcoI and AccI sites, with
B. licheniformis 14353 (21) at the PstI site, and with B.licheniformis 15413 (22) at the HindII, EcoRI and NdeI sites. On the
restriction enzyme map, CK was most similar to that of Blicheniformis 14353, but the first amino acid of the N-terminal
amino acid sequence of CK is alanine rather than glycine, as it
is for the B. licheniformis 14353 protease. These results demonstrate that CK is not identical to subtilisin Carlsberg or its
isoforms.

Answer 1

这段话应该是某篇文献的一部分。
翻译如下：
CK基因与枯草杆菌素Carlsberg及其亚型的限制性内切酶切图谱比较。为了区分CK和枯草杆菌素Carlsberg，进行了5次PCR以消除taqdna聚合酶的错误。
将PCR产物插入pGEM-7Zf（1）载体，转化大肠杆菌JM109。每个含有CK基因PCR产物的质粒用多种限制性内切酶进行酶切，得到了相同的结果。
与图4所示枯草杆菌素嘉士伯及其亚型的限制性内切酶图谱的比较表明，CK基因的限制性内切酶位点与地衣芽孢杆菌NCIB 6816（枯草杆菌素嘉士伯）（9）的PstI、AccI和HindII位点不同。
在NcoI和AccI地点的地衣芽孢杆菌11594（20）和PstI地点的地衣芽孢杆菌14353（21）以及HindII、EcoRI和NdeI地点的地衣芽孢杆菌15413（22）也存在明显的差异。
在限制性内切酶图谱上，CK与blicheniformis14353最为相似，但CK的N端氨基酸序列的第一个氨基酸是丙氨酸而不是甘氨酸，地衣芽孢杆菌14353蛋白酶也是如此。
这些结果表明，CK与枯草杆菌素及其亚型并不完全相同。
最后，温馨提示：在翻译的注意断句，先把句子的主干找出来，最后在加入修饰成分，就可以翻译出来了。
专用词汇需要单独翻译啊。
望采纳~