Question

高分求助，科研论文中翻英~！

目的：探讨在不同成熟状态下对树突状细胞(dendritic cell, DC)分泌的外泌体（exosomes，Dex）的制备、提取及体外功能鉴定。 方法：F344大鼠骨髓来源DC培养6天后分为2组,组一为未成熟树突状细胞组（immature DC,imDC）:直接收集上清；组二为成熟树突状细胞组（mature DC, mDC）: 每孔加入终浓度为1μg/m l 的LPS培养48小时后，收集上清。然后用多步离心法分离得到Dex，流式细胞术检测两组Dex的表型, 酶联免疫吸附测定(ELISA )检测两组Dex与Wistar大鼠骨髓来源的imDC共培养上清的白细胞介素10 ( IL-10)、 白细胞介素12 ( IL -12p70)的含量, 混合淋巴细胞实验（MLR）检测共培养DCs刺激T 细胞增殖能力。结果：与mDex分泌的exosomes相比，imDC分泌的exosomes表面中等表达MHC-Ⅱ类分子、低表达CD80、CD86、CD40共刺激分子,而且与Wistar大鼠骨髓来源的imDC共培养后刺激IL-12p70分泌能力较低（p＜0.05），刺激T淋巴细胞增殖的能力也较mDC分泌的exosomes组弱（p＜0.05）。结论：不同成熟状态下的DC分泌的exosomes功能各异，可能在宿主的免疫反应中发挥不同的作用。

Answer 1

【Google版本】

Explore the different maturation state of dendritic cells (dendritic cell, DC) precursors secrete the outer secretion (exosomes, Dex) the preparation, extraction and identification of in vitro function. Methods: F344 rat bone marrow-derived DC cultured 6 days divided into 2 groups, group one group of immature dendritic cells (immature DC, imDC): directly from the supernatant; Group II for mature dendritic cells (mature DC, mDC): final concentration of each hole by adding 1μg/ml of LPS for 48 hours after culture, collection of supernatant. And then use the multi-step centrifugation isolated Dex, the two groups were detected by flow cytometry phenotype of Dex,
Enzyme-linked immunosorbent assay (ELISA) detected two groups of Dex and Wistar rats were imDC bone marrow-derived culture supernatant IL-10 (IL-10), interleukin-12 (IL-12p70) levels, mixed lymphocyte experiments (MLR) Detection of co-cultured DCs ability to stimulate T cell proliferation. Results: Compared with exosomes secreted mDex compared, imDC secreted exosomes surface expression of MHC-Ⅱ middle-class, low expression of CD80, CD86, CD40 co-stimulatory molecules, but also bone marrow-derived Wistar rats were cultured imDC stimulate IL-12p70 low secretion (p <0.05), to stimulate T lymphocyte proliferation than the capacity of exosomes secreted by mDC weak group (p <0.05). Conclusion: The different mature DC state of exosomes secreted by different functions, may be in the host immune response to play a different role.

【bing必应在线翻译】

Explore different mature state on dendritic cells (dendritic cell, DC) secretion of exosomes (exosomes,-Dex): preparation, extraction and functional characterization. Methods: F344 rats bone marrow-derived DC culture 6 days later divided into 2 groups, group a to immature dendritic cells Group (imDC) immature DCs,: the supernatant of direct collection; Group b to mature dendritic cells group, solo DC, mDC): the concentration of each hole join mallets to 1μg/m l LPs culture after 48 hours to collect the supernatant.And then use multi-step centrifugation separation get-Dex, flow cytometry two groups-Dex phenotype, enzyme linked immunosorbent assay (ELISA) to detect two groups-Dex and Wistar Rat bone marrow-derived imDC total supernatants of interleukin-10 (IL-10), IL-12 (IL-12p70) of content, mixed lymphocyte experiments (MLR) detection co-culture DCs stimulation of t-cell proliferation.Results: and mDex secretion of exosomes imDC secretion than exosomes surface expression of MHC class medium molecules, low expression CD80, CD86, CD40 Costimulatory molecules, and unlike Wistar Rat bone marrow-derived imDC co-culture secretion capacity after stimulation IL-12p70 (p < lower 0.05) to stimulate the T lymphocyte proliferation have mDC secretion of exosomes group weak (p < 0.05).Conclusion: different mature state DC secretion of exosomes functionality varies, may be in the host immune reactions play different roles.

Answer 2

Objective: To explore the different state of mature dendritic cells (dendritic cell, DC) precursors secrete the outer secretion (exosomes, Dex) the preparation, extraction and identification of in vitro function. Methods: F344 rat bone marrow-derived DC cultured 6 days divided into 2 groups, group one group of immature dendritic cells (immature DC, imDC): directly from the supernatant; Group II for mature dendritic cells (mature DC, mDC): final concentration of each hole by adding 1μg/ml of LPS for 48 hours after culture, collection of supernatant. And then use the multi-step centrifugation isolated Dex, the two groups were detected by flow cytometry Dex phenotype, enzyme-linked immunosorbent assay (ELISA) detected two groups of Dex and Wistar rats were imDC bone marrow-derived culture supernatant of IL - 10 (IL-10), interleukin-12 (IL-12p70) levels, experimental mixed lymphocyte (MLR) Detection of co-cultured DCs ability to stimulate T cell proliferation. Results: Compared with exosomes secreted mDex compared, imDC secreted exosomes surface expression of MHC-Ⅱ middle-class, low expression of CD80, CD86, CD40 co-stimulatory molecules, but also bone marrow-derived Wistar rats were cultured imDC stimulate IL-12p70 low secretion (p <0.05), to stimulate T lymphocyte proliferation than the capacity of exosomes secreted by mDC weak group (p <0.05). Conclusion: The different mature DC state of exosomes secreted by different functions, may be in the host immune response to play a different role.

Answer 3

Objective: to study in different mature state on dendritic cells (dendritic cell, DC) secretion of exosomes (exosomes,-Dex): preparation, extraction and functional characterization. Methods: F344 rats bone marrow-derived DC culture 6 days later divided into 2 groups, group a to immature dendritic cells Group (imDC) immature DCs,: the supernatant of direct collection; Group b to mature dendritic cells group, solo DC, mDC): the concentration of each hole join mallets to 1μg/m l LPs culture after 48 hours to collect the supernatant.And then use multi-step centrifugation separation get-Dex, flow cytometry two groups-Dex phenotype, enzyme linked immunosorbent assay (ELISA) to detect two groups-Dex and Wistar Rat bone marrow-derived imDC total supernatants of interleukin-10 (IL-10), IL-12 (IL-12p70) of content, mixed lymphocyte experiments (MLR) detection co-culture DCs stimulation of t-cell proliferation.Results: and mDex secretion of exosomes imDC secretion than exosomes surface expression of MHC class medium molecules, low expression CD80, CD86, CD40 Costimulatory molecules, and unlike Wistar Rat bone marrow-derived imDC co-culture secretion capacity after stimulation IL-12p70 (p < lower 0.05) to stimulate the T lymphocyte proliferation have mDC secretion of exosomes group weak (p < 0.05).Conclusion: different mature state DC secretion of exosomes functionality varies, may be in the host immune reactions play different roles.