Question

能不能帮我翻译一下这些东西啊 各位大侠 谢谢了

Research Proposal August 2009
in connection with the employment of Dr XXX
丹参（Radix Salviae Miltiorrhizae(拉)）CCR和COMT基因
与次生代谢产物（Secondary metabolites）的关系
1. Introduction and motivation
国内外研究进展
 CCR 活性严重下调的转基因烟草不仅显示了木质素含量的显著下降, 并伴随着不正常生长: 如植株矮小, 畸形叶片, 导管塌陷等。
 抑制COMT表达，转基因植物木质素中S组分明显降低，G型木质素影响较小。S/G比值下降。Ma等认为用维管组织特异表达启动子可以调节COMT的转录。
 目前, 除对极少的木质素建立了结构模型外, 其他很多木质素空间结构和代谢途径还不完全清楚。
 CCR基因和COMT基因在经济植物中的研究较多，但对于它们在丹参中次生代谢物积累作用的研究目前还没有报道。

选题依据
 肉桂酰辅酶A还原酶（CCR）和咖啡酸-3-O-甲基转移酶（COMT）是木质素合成中的关键酶。
 木质素是苯丙烷代谢途径的重要产物，同时，丹参中的药用成分多为苯丙烷代谢途径的产物。

2. Research objectives
研究内容
 丹参CCR 基因和COMT基因的RNA-i载体的 构建及转化
 丹参中CCR基因和COMT基因干涉株系的分子水平检测（DNA水平和RNA水平）
 对丹参CCR基因和COMT基因干涉成功株系的次生代谢产物含量的检测
 总木质素，单体木质素含量检测以及木质素的显微结构
拟解决的关键问题
 获得CCR- RNAi和COMT- RNAi丹参转化体

 转化体次生代谢物质和木质素含量的检测

3. Methods and Data

获得基因片段

可行性分析
 理论分析：木质素是苯丙烷代谢途径的重要产物，同时，丹参中的药用成分多为苯丙烷代谢途径的产物。
 方法分析：本研究所要用到的实验技术如：荧光定量PCR技术都已经成熟，丹参的外源基因转化体系已建立，并在本实验室有较广泛的应用，丹参HPLC成份检测体系已建立。
 仪器分析：实验相关的仪器设备等均已具备，且易操作。

4. Project schedule
2010.7-2011.1 查阅文献及相关资料

2011.1-2011.5 CCR基因和COMT基因的遗传转化

2011.4-20011.9 转基因植株的获得

2011.9-2012.1 转基因植株分子检测、生理指标检测

2012.1-2012.3 撰写论文

2012.3-2012.5 论文修改及答辩

5. Collaborations
.
6. Finance

7. Expected outcome
References
谢谢各位了 我不想要google上直接贴的 要汇集各位的知识 啊 谢谢啦

Answer 1

Research Proposal August 2009
in connection with the employment of Dr XXX
丹参（Radix Salviae Miltiorrhizae(拉)）CCR和COMT基因
与次生代谢产物（Secondary metabolites）的关系
1. Introduction and motivation
国内外研究进展
 CCR 活性严重下调的转基因烟草不仅显示了木质素含量的显著下降, 并伴随着不正常生长: 如植株矮小, 畸形叶片, 导管塌陷等。
 抑制COMT表达，转基因植物木质素中S组分明显降低，G型木质素影响较小。S/G比值下降。Ma等认为用维管组织特异表达启动子可以调节COMT的转录。
 目前, 除对极少的木质素建立了结构模型外, 其他很多木质素空间结构和代谢途径还不完全清楚。
 CCR基因和COMT基因在经济植物中的研究较多，但对于它们在丹参中次生代谢物积累作用的研究目前还没有报道。

选题依据
 肉桂酰辅酶A还原酶（CCR）和咖啡酸-3-O-甲基转移酶（COMT）是木质素合成中的关键酶。
 木质素是苯丙烷代谢途径的重要产物，同时，丹参中的药用成分多为苯丙烷代谢途径的产物。

2. Research objectives
研究内容
 丹参CCR 基因和COMT基因的RNA-i载体的 构建及转化
 丹参中CCR基因和COMT基因干涉株系的分子水平检测（DNA水平和RNA水平）
 对丹参CCR基因和COMT基因干涉成功株系的次生代谢产物含量的检测
 总木质素，单体木质素含量检测以及木质素的显微结构
拟解决的关键问题
 获得CCR- RNAi和COMT- RNAi丹参转化体

 转化体次生代谢物质和木质素含量的检测

3. Methods and Data

获得基因片段

可行性分析
 理论分析：木质素是苯丙烷代谢途径的重要产物，同时，丹参中的药用成分多为苯丙烷代谢途径的产物。
 方法分析：本研究所要用到的实验技术如：荧光定量PCR技术都已经成熟，丹参的外源基因转化体系已建立，并在本实验室有较广泛的应用，丹参HPLC
Research Proposal August 2009

in connection with the employment of Dr XXX

Danshen (Radix Salviae Miltiorrhizae (la)) CCR and COMT gene

And secondary metabolites (Secondary metabolites) the relationship between the

1. Introduction and motivation

Progress at home and abroad

 CCR severely reduced activity of transgenic tobacco not only shows the lignin content decreased significantly, accompanied by abnormal growth of: such as plants stunted, deformed leaves and other catheter collapse.

 COMT inhibited the expression of lignin in transgenic plants significantly reduced S component, G-type lignin less affected. S / G ratio decreased. Ma so that the use of vascular tissue-specific expression of COMT promoter can regulate transcription.

 At present, very little lignin in addition to the establishment of a structural model, the spatial structure of lignin and many other metabolic pathways are not entirely clear.

 CCR gene and COMT gene in the study of economic plants are more, but their accumulation of secondary metabolites in Danshen the role of research is currently still not reported.

Based on topics of

 cinnamon coenzyme A reductase (CCR) and caffeic acid-3-O-methyltransferase (COMT) is a key enzyme in lignin synthesis.

 lignin is a major metabolic pathway of benzene, a product of propane, while the medicinal ingredients in Danshen are mostly the product of phenylpropanoid pathway.

 

2. Research objectives

The study

 Salvia and the COMT gene CCR gene RNA-i vector and transformation of

 Danshen in the CCR gene and COMT gene interfere with detection of strains at the molecular level (DNA level and RNA level)

 right Salvia CCR gene and COMT gene interfere with the success of strains of the Secondary Metabolites Detection

 Total lignin, lignin monomer lignin content detection and Microstructure

The key issues to be addressed

 be CCR-RNAi and COMT-RNAi transformants Salvia

 transformants secondary metabolites and lignin content of the test

3. Methods and Data

                             Access to genetic fragments

 

Feasibility Analysis

 theoretical analysis: Lignin is a major metabolic pathway of benzene, a product of propane, while the medicinal ingredients in Danshen are mostly the product of phenylpropanoid pathway.

 Methods: The Institute of use to the experimental techniques such as: fluorescent quantitative PCR technology is mature, Salvia exogenous gene transfer system has been established, and in this laboratory have a broader application, Salvia HPLC

Answer 2

Research Proposal August 2009
in connection with the employment of Dr XXX
Danshen (Radix Salviae Miltiorrhizae (la)) CCR and COMT gene
And secondary metabolites (Secondary metabolites) the relationship between the
1. Introduction and motivation
Progress at home and abroad
 CCR severely reduced activity of transgenic tobacco not only shows the lignin content decreased significantly, accompanied by abnormal growth of: such as plants stunted, deformed leaves and other catheter collapse.
 COMT inhibited the expression of lignin in transgenic plants significantly reduced S component, G-type lignin less affected. S / G ratio decreased. Ma so that the use of vascular tissue-specific expression of COMT promoter can regulate transcription.
 At present, very little lignin in addition to the establishment of a structural model, the spatial structure of lignin and many other metabolic pathways are not entirely clear.
 CCR gene and COMT gene in the study of economic plants are more, but their accumulation of secondary metabolites in Danshen the role of research is currently still not reported.
Based on topics of
 cinnamon coenzyme A reductase (CCR) and caffeic acid-3-O-methyltransferase (COMT) is a key enzyme in lignin synthesis.
 lignin is a major metabolic pathway of benzene, a product of propane, while the medicinal ingredients in Danshen are mostly the product of phenylpropanoid pathway.

2. Research objectives
The study
 Salvia and the COMT gene CCR gene RNA-i vector and transformation of
 Danshen in the CCR gene and COMT gene interfere with detection of strains at the molecular level (DNA level and RNA level)
 right Salvia CCR gene and COMT gene interfere with the success of strains of the Secondary Metabolites Detection
 Total lignin, lignin monomer lignin content detection and Microstructure
The key issues to be addressed
 be CCR-RNAi and COMT-RNAi transformants Salvia
 transformants secondary metabolites and lignin content of the test
3. Methods and Data
Access to genetic fragments

Feasibility Analysis
 theoretical analysis: Lignin is a major metabolic pathway of benzene, a product of propane, while the medicinal ingredients in Danshen are mostly the product of phenylpropanoid pathway.
 Methods: The Institute of use to the experimental techniques such as: fluorescent quantitative PCR technology is mature, Salvia exogenous gene transfer system has been established, and in the laboratory have a broader application of Salvia HPLC detection components system has been created.
 Instrument Analysis: Experimental and other related equipment are in place, and easy to operate.
4. Project schedule
2010.7-2011.1 access to documentation and related information

2011.1-2011.5 CCR gene and COMT gene in genetic transformation of
2011.4-20011.9 Transgenic Plants
2011.9-2012.1 molecular detection of genetically modified plants, physiological indicators of test
2012.1-2012.3 writing papers
2012.3-2012.5 papers modification and defense
5. Collaborations
.
6. Finance
7. Expected outcome
References