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2.10.Stabilitystudies2.10.1.StoragestabilityDoxorubicinloadednanoparticleswerestoreda...
2.10. Stability studies
2.10.1. Storage stability
Doxorubicin loaded nanoparticles were stored at 4 ◦C and were studied for particle size, zeta potential and drug content up to 1 month. Particle size and zeta potential were determined by particle size analyzer and DOX content was determined using UV/vis spectrophotometer.
2.10.2. Effect of pH and ionic concentration of medium
For the determination of effect of pH on the stability, DXGP were incubated in different pH medium (pH 3–11). Nanoparticles incubated in phosphate buffer saline pH 7.4 were taken as control. Absorbance was measured after 24 h and compared with absorbance of control. The effect of ionic strength on the stability of DXNP was determined by incubating the nanoparticles in deionised water containing varying concentrations (0.1–2 M) of sodium chloride
(NaCl).
2.10.3. Serum stabilityof DXGP
Serum stability of DXGP was evaluated at two different serum concentration levels (10%, v/v, and 100% serum). Nanoparticles were dispersed in serum and particlessize, zeta potential and absorbance were measured at different time intervals up to 72 h.
2.11. Statistical analysis
All the experiments were performed in triplicates. Data are expressed as Mean ± SD (standard deviation) (n = 3). The values of in vitro drug release, cytotoxicity and stability studies were statistically analyzed using student’s t-test. Statistical significance levelwas set at p < 0.05.
3. Result and discussion
3.1. Preparation and optimization of gold nanoparticles Gold nanoparticles were prepared by chemical reduction method using xanthan gum as reducing agent. The role of gumconcentration was studied by using gum solution in the range of 0.5–2 mg/ml containing 10 mM of HAuCl4 concentration (Fig. 1a).
The absorbance in the UV spectrum which describes the reduc-
tion of GNP, was found to be directly related to gum concentration.
The intensity of the absorption band gradually increased and the
maximum intensity of absorption was attained at 1.5 mg/ml which
indicated the maximum gold nanoparticle concentration. 展开
2.10.1. Storage stability
Doxorubicin loaded nanoparticles were stored at 4 ◦C and were studied for particle size, zeta potential and drug content up to 1 month. Particle size and zeta potential were determined by particle size analyzer and DOX content was determined using UV/vis spectrophotometer.
2.10.2. Effect of pH and ionic concentration of medium
For the determination of effect of pH on the stability, DXGP were incubated in different pH medium (pH 3–11). Nanoparticles incubated in phosphate buffer saline pH 7.4 were taken as control. Absorbance was measured after 24 h and compared with absorbance of control. The effect of ionic strength on the stability of DXNP was determined by incubating the nanoparticles in deionised water containing varying concentrations (0.1–2 M) of sodium chloride
(NaCl).
2.10.3. Serum stabilityof DXGP
Serum stability of DXGP was evaluated at two different serum concentration levels (10%, v/v, and 100% serum). Nanoparticles were dispersed in serum and particlessize, zeta potential and absorbance were measured at different time intervals up to 72 h.
2.11. Statistical analysis
All the experiments were performed in triplicates. Data are expressed as Mean ± SD (standard deviation) (n = 3). The values of in vitro drug release, cytotoxicity and stability studies were statistically analyzed using student’s t-test. Statistical significance levelwas set at p < 0.05.
3. Result and discussion
3.1. Preparation and optimization of gold nanoparticles Gold nanoparticles were prepared by chemical reduction method using xanthan gum as reducing agent. The role of gumconcentration was studied by using gum solution in the range of 0.5–2 mg/ml containing 10 mM of HAuCl4 concentration (Fig. 1a).
The absorbance in the UV spectrum which describes the reduc-
tion of GNP, was found to be directly related to gum concentration.
The intensity of the absorption band gradually increased and the
maximum intensity of absorption was attained at 1.5 mg/ml which
indicated the maximum gold nanoparticle concentration. 展开
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2015-03-11 · 知道合伙人教育行家
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2.10。稳定性研究
2.10.1。储存稳定性
阿霉素纳米粒分别存储在4 C和研究◦粒度,Zeta电位、药物含量高达1个月。粒径和Zeta电位测定、粒度分析仪和DOX使用紫外/可见分光光度法测定含量。
参赛人员。pH值和介质的离子浓度的影响
的pH值对稳定性的影响,测定了在不同pH介质中,dxgp培养(pH 3–11)。纳米粒子孵育在磷酸盐缓冲液pH为7.4作为控制。测定24小时后,与对照相比吸光度吸光度。通过将纳米颗粒在含有不同浓度的去离子水测定的离子强度对dxnp稳定性的影响(0.1–2米)的氯化钠
(NaCl)。
2.10.3。dxgp血清稳定性
在两个不同的血清浓度进行dxgp血清稳定性(10%,V / V,100%血清)。纳米粒子分散在血清和particlessize,在不同的时间间隔为72小时。测定Zeta电位和吸光度
2.11。统计分析
所有试验均进行了一式三份。数据表示的意思±SD(标准差)(n = 3)。值的体外药物释放,细胞毒性和稳定性研究进行统计学分析,采用t检验。在P<0.05组统计意义上显著fi。
3。结果与讨论
3.1。采用黄原胶作为还原剂的化学还原方法制备金纳米粒子和金纳米粒子的制备优化。gumconcentration的作用是通过使用胶溶液在2毫克/毫升0.5–含有HAuCl4溶液的浓度范围内研究了10 mm(图1A)。
其中介绍了紫外光谱的吸光度的减少—
对国民生产总值,被认为是胶的浓度直接相关。
的吸收峰的强度逐渐增加,
吸收强度最大值达到1.5毫克/毫升
显示的最大的金纳米粒子的浓度。
2.10.1。储存稳定性
阿霉素纳米粒分别存储在4 C和研究◦粒度,Zeta电位、药物含量高达1个月。粒径和Zeta电位测定、粒度分析仪和DOX使用紫外/可见分光光度法测定含量。
参赛人员。pH值和介质的离子浓度的影响
的pH值对稳定性的影响,测定了在不同pH介质中,dxgp培养(pH 3–11)。纳米粒子孵育在磷酸盐缓冲液pH为7.4作为控制。测定24小时后,与对照相比吸光度吸光度。通过将纳米颗粒在含有不同浓度的去离子水测定的离子强度对dxnp稳定性的影响(0.1–2米)的氯化钠
(NaCl)。
2.10.3。dxgp血清稳定性
在两个不同的血清浓度进行dxgp血清稳定性(10%,V / V,100%血清)。纳米粒子分散在血清和particlessize,在不同的时间间隔为72小时。测定Zeta电位和吸光度
2.11。统计分析
所有试验均进行了一式三份。数据表示的意思±SD(标准差)(n = 3)。值的体外药物释放,细胞毒性和稳定性研究进行统计学分析,采用t检验。在P<0.05组统计意义上显著fi。
3。结果与讨论
3.1。采用黄原胶作为还原剂的化学还原方法制备金纳米粒子和金纳米粒子的制备优化。gumconcentration的作用是通过使用胶溶液在2毫克/毫升0.5–含有HAuCl4溶液的浓度范围内研究了10 mm(图1A)。
其中介绍了紫外光谱的吸光度的减少—
对国民生产总值,被认为是胶的浓度直接相关。
的吸收峰的强度逐渐增加,
吸收强度最大值达到1.5毫克/毫升
显示的最大的金纳米粒子的浓度。
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这是百度翻译的吧。基本上没有逻辑性啊~
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