Question

求高手翻译成英文，不要用软件，在线急等

为了明确光皮桦BlSPL2基因组结构，为其SNP分析提供基础，本研究根据cDNA序列设计特异引物，以光皮桦基因组总DNA为模板进行了PCR扩增和测序分析，并将其与cDNA序列进行比对。结果表明，BlSPL2基因组全长3554bp，含有2个内含子，3个外显子，第一个内含子长1005bp，第二个内含子长624bp，3个外显子分别长416，134和620bp长，在第三个外显子处（3025-3044）存在miR156位点，5’-UTR长485bp，3’-UTR长169bp。。在此基础上，对其第二个内含子和3’-UTR区约850bp设计引物克隆基因片段用于SNP分析，以75个来自不同种源的无性系为材料，克隆相应的片段。利用MEGA5.0软件对光皮桦75株基因型个体的序列进行比对和分析，利用DNsp5检测到103个单核苷酸多态性位点，SNP频率为1/8bp.在这些SNPs中，有96个是二态SNPs，其中个52属于转换，44个属于颠换。对SPL2基因内SNPs进行的连锁不平衡分析结果显示，随着核苷酸序列长度的增加，SNPs的连锁不平衡在基因内部逐渐增加，但较平缓。研究结果为光皮桦SPL基因的连锁不平衡作图及其基因辅助光皮桦进行控制开花的分子育种提供了理论依据。
语句翻译一下好了，专业术语自己会加，求大神翻译，就像第一句，只要翻译为了明确。。。的结构，为。。。分析提供基础。后面我翻好了，只要翻为了明确光皮桦BlSPL2基因组结构，为其SNP分析提供基础，本研究根据cDNA序列设计特异引物，以光皮桦基因组总DNA为模板进行了PCR扩增和测序分析，并将其与cDNA序列进行比对。结果表明，BlSPL2基因组全长3554bp，含有2个内含子，3个外显子，第一个内含子长1005bp，第二个内含子长624bp，3个外显子分别长416，134和620bp长，在第三个外显子处（3025-3044）存在miR156位点，5’-UTR长485bp，3’-UTR长169bp

Answer 1

In order to make genome organization of Betula luminifera BISPL2, provide basis for its SNP analysis, this research according to cDNA sequence designs pecullar primer, takes total DNA of Betula luminifera genome as template to conduct PCR amplificaton and sequencing analysis, and compare with cDNA sequence. The result shows that overall length of BISPL2 genome is 3554bp, it contains 2 intron, 3 exon. Length of the first intron is 1005bp, of the second is 624bp. Length of the 3 exon are 416, 134 and 620bp, respectively. At the third exon (3025-3044) exists miR156 locus, length of 5’-UTR is 485bp and of 3’-UTR is 169bp. On this basis, for its second intro and 3’-UTR area about 850bp, designs primer clone gene segment used for SNP analysis, clone corresponding segment taking 75 clones from different provenance as materials. Using MEGA5.0 to compare and analyze sequence of 75 genetype indivudual of Betula luminifera. By using of DNsp5, detect 103 single nucleotide polymorphisms. SNP frequency is 1/8bp. Of these SNPs, 96 are dimor-phism SNPs, among which 52 blong to transition, 44 belong to transversion. The result of linkage disequilibrim analysis to SNPs in SPL2 gene shows that along with the increase of nucleotide sequence length, linkage disequilibrim of SNPs grandually increases in the gene, but gently. The research results provide theoretical basis for linkage disequilibrim mapping of Betula luminifera SPL gene and its genes assisting Betula luminifera control breeding of flowering molecular.

我这山蚂弊是纯物冲手工逗族翻译的

追问

蛋碎，我已经给别人分了

追答

给就给呗，稀罕你这点分啊，我答题从来不看分的，只是看你这个我可以翻译的来才做的，不用这么小瞧人吧。

追问

我不是这个意思，只是感觉过意不去，我这个人太敏感的，我是天秤座的，别人帮过我我都铭记在心的

追答

这根敏感没有关系，我有工作的，没事的时候才会上来看看，有能回答的就回答，我不会瞎回答的。呵呵没事，以后有问题可以问我啊，免费的，(*^__^*) 嘻嘻……

Answer 2

别伏消的不会，只能机器翻译了，先看别人的敏郑，实在没有的话你缺拿知在看我的就行
Clear light skin the birch BlSPL2 genome structure for SNP analysis based on this study, specific primers were designed according to the cDNA sequence, light birch genomic DNA as template for PCR amplification and sequencing analysis, and cDNA sequencecompared. The results show that the 3554bp BlSPL2 genomic full-length, containing two introns, exon 3, containing sub-length 1005bp, the second intron of 624bp, three exons, respectively, 416,134 and 620bp long miR156 sites exist in the third exon (3025-3044), 5'-UTR of length 485bp 3'-UTR length of 169bp. . On this basis, its second intron and the 3'-UTR is about 850bp primers were designed objects cloning of gene fragments for SNP analysis, 75 clones from different source materials, cloning the corresponding fragment. Light birch, MEGA5.0 software 75 genotype sequences for comparison and analysis, the use DNsp5 detected 103 single nucleotide polymorphisms (SNPs), the SNP frequency as 1/8bp these SNPs 96 of the SNPs of the two states, which 52 belongs to the transformation, 44 were transversions. The SPL2 gene within SNPs in linkage disequilibrium analysis showed that with the increase in the length of nucleotide sequence, linkage disequilibrium of SNPs in the gene internal gradually increased, but more gentle. The results for the light Betula SPL gene linkage disequilibrium mapping and gene auxiliary light Betula molecular breeding provides a theoretical basis for the control of flowering.

追问

机器翻译我自己也会，翻译的好我追加50

追答

那你就先等等吧，我只是为了帮助别人，尽自己最大的努力

Answer 3

亲，您的内容太专业了呀，建议用机器翻译出来，然后找个专业人士修改一下

追问

没有专业人士啊，大哥帮忙改一下，只要语句翻译一下，专业术语我自己会加的

追答

好吧，我来尝试一下
为了明确光皮桦BlSPL2基因组结构，为其SNP分析提供基础，本研究根据cDNA序列设计特异引物，以光皮桦基因组总DNA为模板进行了PCR扩增和测序分析，并将其与cDNA序列进行比对。
In order to find out the genome structure of 皮桦BlSPL2 and provide the basis for the SNP analysis，according to the cDNA sequence this study design特异引物，
额，翻译不下去了……
这是论文吧？建议你还是赵老师求助一下吧，不好意思啦

追问

。。。老师也搞不定

追答

%>_<%
专业术语太多了，是在进行不下去呀
要不你就去图书馆找下类似的外文文献，最好有中英对照的

追问

我有一篇范文的，要不你看一下，我发你，他的摘要和我基本一样的，就语句不一样，术语都一样的

追答

一会我有事，要不你发过来，回头忙完了，给你看一下，呵呵

追问

怎么发给你

追答

用百度 hi 我吧