Question

请大家帮帮忙，翻译一段医学英语文章，很急的，谢谢

Given that palmitoylation enhances protein trafficking to caveolae [28,46], it is likely that the inhibition in palmitoylation of caveolin-1 mediated by H2O2 is responsible for the observed decrease in targeting to membrane rafts. However, it is also possible that the converse mechanism is important, namely that the observed decrease in palmitoylation is a consequence (not the cause) of impaired targeting of caveolin-1 to membrane rafts in the presence of H2O2. Indeed, with few exceptions [47], protein palmitoylation occurs primarily at the plasma membrane, where palmitoyl transferase activity is concentrated [48,49]. Newly synthesized caveolin-1 might be incorporated into detergent-resistant membranes in the Golgi apparatus [50]. We have previously shown that inhibition of trafficking of newly synthesized caveolin-1 to the plasma membrane by Brefeldin A or monensin results in a marked decrease in caveolin-1 palmitoylation, indicating that caveolin-1 palmitoylation occurs after the trans-Golgi compartment [31]. Thus we cannot exclude the possibility that oxidative stress, by an unknown mechanism, alters the trafficking of nascent caveolin-1, which therefore does not reach its membrane site of palmitate incorporation.

Answer 1

鉴于palmitoylation提高蛋白质贩卖的小窝[ 28,46 ] ，很可能抑制在palmitoylation的小凹- 1介导的过氧化氢是负责观察减少，针对膜竹排。不过，也有可能是逆向的机制是非常重要的，即观察到的减少palmitoylation是一个后果， （而不是事业）针对受损的小凹- 1至膜排在在场的过氧化氢。事实上，除了少数例外[ 47 ] ，蛋白质palmitoylation发生主要是在质膜，其中棕榈转移酶活性，是集中[ 48,49 ] 。新合成凹- 1可能会被纳入洗涤剂耐膜，在高尔基器具[ 50 ] 。我们先前已表明，抑制贩运的新合成凹- 1至质膜由brefeldin A或莫能菌素的结果在显着减少凹- 1 palmitoylation ，表明凹- 1 palmitoylation后，会出现跨高尔基间[ 31 ] 。因此，我们不能排除这样一种可能性，即氧化应激，由一个未知的机制，改变了贩运新生的小凹- 1 ，因此，没有达到其膜网站的棕榈团。