哪位好心且英语好的人能帮我翻译一下这段文字呢?很着急,谢谢大家,拜托了
目的利用PCR扩增法制备出使用方便、条带清晰的DNA分子量标准,摸索并优化其制备条件,为产业化生产提供参考。方法运用PCR技术分别扩增不同大小的特异性条带,确定其浓度,按...
目的 利用PCR扩增法制备出使用方便、条带清晰的DNA分子量标准,摸索并优化其制备条件,为产业化生产提供参考。方法 运用PCR技术分别扩增不同大小的特异性条带,确定其浓度,按比例混合后组成所需要的DNA分子量标准。结果 产品为六条分子量单一的线状DNA条带,用于确定100-600bp的双链DNA分子的大小,并附有上样缓冲液,使用方便快捷。通过电泳与国内外知名品牌的类似产品对比,本产品在条带亮度、均一性和背景方面均应达到同等水平。结论 PCR扩增法可以获得优良的DNA分子量标准。
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Purpose of the use of PCR prepared by ease of use, with clear of the DNA molecular weight standards, and to optimize their exploration of the conditions for the industrial production of reference. Methods were amplified PCR technology for different sizes of the specificity of the band, its concentration, in proportion to the needs of mixed composition of the DNA marker. The results for the six products of a single linear DNA molecular weight of the belt, used to determine 100-600 bp of double-stranded DNA molecule the size, with a sample buffer, easy to use fast. Electrophoresis and through domestic and international famous brands of similar products contrast, the product of the zone in brightness, uniformity and background should be to achieve the same level. Conclusion of PCR method can be an excellent DNA marker.
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