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(3)求论文翻译,急!!!

2.3. Colony count analysis for the candidacidal activity of di-K19Hc
100 ll of a predetermined concentration of di-K19Hc was mixed
with 1 ml aliquots of yeast-phase C. albicans (3 · 107 colony forming
units/ml, CFU/ml), in 10 mM sterile sodium phosphate buffer (pH
7.4). The mixtures, in final volumes of 1.1 ml, were then incubated in
a shaking incubator at 30 C, and 100 ll aliquots were removed at predetermined
intervals. Each of the aliquots directly or after dilution was
plated on SDB agar consisting of 1% agarose and 3% SDB powder in
10 mM sodium phosphate buffer, at neutral pH. The resultant colonies
were then counted, after being incubated overnight at 30 C.
2.4. Potassium efflux
The K+ efflux from the di-K19Hc-treated C. albicans cells was measured
with An Istek model 735 pH/ISE meter, fitted with a potassium
ion electrode (pHoenix Electrode, USA), as was described in the study
of Orlov et al. [22]. In brief, the C. albicans KCTC7122 cells were cultured
overnight in 50 ml of SDB, at 30 C. The cells were washed three
times with 10 mM sodium phosphate buffer, pH 7.4, and resuspended
at a concentration of 3.5 · 107 cells/ml in the same buffer. One milliliter
aliquots of these C. albicans suspensions were then incubated with
100 ll of di-K19Hc (1 mg/ml in 0.01% acetic acid) at 30 C, for predetermined
times. After a 30-s spindown at 12000 rpm, 9 ml of distilled
water was added to the supernatant of each of the samples. The total
K+ efflux was then determined, as the concentration of K+ released
from the C. albicans after the disruption of the samples via prolonged
sonication (3 min at 60% power: Gera¨te-Type UW 2200, Bandelin
Electronic, Berlin, Germany). These experiments were repeated five
times, and the mean values were utilized in the construction of the
graph. The percent K+ release was then calculated, according to the
following equation: K+ release (%) = ([K+] of sample
[K+] of peptide-
free control)/([K+] of 100% control
[K+] of peptide-free control)
· 100.

Answer 1

2.3 。菌落计数分析，为candidacidal活动涤k19hc
100名当地雇员的一个预定的浓度涤k19hc好坏参半
与1毫升aliquots酵母相白念珠菌（ 3 107集落形成
单位/毫升，对CFU /毫升） ，在10毫米无菌磷酸钠缓冲液（ pH
7.4 ） 。该混合物，在最后卷一点一毫升，然后在孵育
一摇头孵化器在30 C和100名当地雇员aliquots被拆除预定
间隔。每一项aliquots直接或稀释后，被
镀对康体发展局的琼脂构成的1 ％琼脂糖和3 ％ ，康体发展局的粉末在
10毫米钠磷酸盐缓冲液，在中性pH 。由此产生的殖民地
当时清点后，孵育，一夜之间在30长
2.4 。钾排
的K +外流的直接投资- k19hc治疗白色念珠菌细胞测量
与一istek模型735 pH值/伊势米，装有一钾
离子电极（凤凰电极，美国） ，正如所描述的研究
对orlov等人。 [ 22 ] 。简单来说，白色念珠菌kctc7122细胞培养
一夜之间在50毫升的康体发展局，在30三，细胞洗涤3
倍，与10毫米钠磷酸盐缓冲液， pH值7.4 ，和resuspended
在浓度三点五一零七细胞/毫升，在相同的缓冲区。一毫升
aliquots这些白色念珠菌悬浮液，然后孵育
100名当地雇员的涤k19hc （ 1毫克/毫升，在0.01 ％ ，乙酸）的30 C ，预定
倍。后30 - S的spindown在12000 rpm的，九毫升蒸馏
水被添加到上清液中的每个样本。总
钾离子流出，当时确定的，由于浓度的钾离子释放
从白色念珠菌后中断的样品经长时间
超声波（ 3分钟在60 ％功率：格拉¨特型威斯康星大学2200年，班得利
电子，柏林，德国） 。这些实验重复5
倍，平均值分别利用在建造该
图。该％ ，钾离子释放，当时的计算方法，根据该
以下方程：钾离子释放（ ％ ） = （ [钾]样本[钾]肽
自由控制） / （钾100 ％的控制[钾]肽自由控制）
100 。
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