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Non-contingentmethadministrationandobject-in-placerecognitionmemory.Ratsreceived4-mg/... Non-contingent meth administration and object-in-placerecognition memory. Rats received 4-mg/kg injections (IP) of non-contingent methamphetamine hydrochloride (Sigma-Aldrich, StLouis, Missouri, USA) or saline at 2 h intervals in their home cage(see Fig. 1 for time line). Following this regimen, rats were placedinto an abstinence period in which they were handled daily. Ondays 5 and 6 of abstinence, rats were habituated to the object-in-place behavioral apparatus for 5 min without objects and on day 7,rats were tested for object-in-place. Testing occurred on a roundwood open field (98 cm diameter, 3.5 cm thickness, 65 cm abovethe floor) painted gray. A video camera positioned above the fieldrecorded the sessions. During the familiarization phase (i.e., samp-ling phase), rats were placed on the apparatus for 5 min with 4distinct objects positioned 15 cm apart on the round field. A 3 minmemory test was conducted 90 min later by placing the rat in theapparatus with the same object, except that the position of twoobjects was changed. The objects used were chosen based on pre-liminary research with naïve rats demonstrating that the objectsengendered similar exploration and consisted of combinations of aPVC pipe (6.4×3.8cm2), a paint roller (7.5×2.5cm2), a light bulb(8.9 cm), and a plastic bottle (12 cm; Reichel et al., 2012b).
The objects that stayed in the same locations vs. the objects that weremoved to a novel location were counterbalanced as much as thesample size allowed and matched between meth and saline groups.
All objects and the apparatus were wiped down with 70% isopropylalcohol between uses.
To test modafinil’s impact on object-in-placememory, rats received a vehicle or modafinil (100 mg/kg) injec-tion immediately after the familiarization session resulting in fourdistinct groups:
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匿名用户
2013-12-29
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非然甲基管理和对象在placerecognition内存。大鼠接受4-mg/kg注射非偶然甲基苯丙胺盐酸盐( Sigma-Aldrich公司, StLouis ,Missouri,美国)或生理盐水在2小时的时间间隔在其家笼(IP)的(参见图1为时间线)。根据这一方案,大鼠placedinto中,他们每天处理的禁欲期。 Ondays 5,6禁欲,大鼠无物体习惯于对象就地行为装置5分钟,并在第7天,将大鼠进行了测试对象的就地。测试发生在一个圆木开放领域(98厘米直径的,3.5厘米厚度, 65厘米abovethe楼)涂上灰色。一台摄像机定位在fieldrecorded会议。在熟悉阶段(即,桑普灵相) ,大鼠放置在5分钟的设备与4distinct对象定位15厘米除了在团场。 A 3 minmemory测试通过将大鼠在theapparatus与同一个对象,只是twoobjects的位置被改变进行90分钟后。根据预先初步的研究被选为与幼稚大鼠证明该objectsengendered类似勘探和包括APVC管的组合( 6.4× 3.8平方厘米) ,涂料辊( 7.5× 2.5平方厘米) ,灯泡( 8.9厘米使用的对象) ,以及塑料瓶(12厘米; 。 Reichel等人, 2012B ) 。那住在相同的位置与该weremoved一种新的位置的对象的对象被抵消高达thesample尺寸允许和甲基组和生理盐水组之间的匹配。所有对象和设备进行擦拭与使用之间70 %异丙醇。为了测试的对象在placememory莫达非尼的影响,大鼠接受车辆或莫达非尼( 100毫克/千克)进样的熟悉会话导致fourdistinct组后:
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匿名用户
2013-12-28
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非偶然的方法管理…
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匿名用户
2013-12-29
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文去呃呃呃去
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