Question

请高手帮我翻译下面的文章。。。不要网上在线翻译的（不给分），要高手亲自翻译的，翻译得好另外加高分(4)

During the second stage of subtraction, samples A and B (having different ligated adaptors) are mixed and allowed to reassociate. This leads to the creation of novel molecules from the subtracted ss-DNA that are by definition asymmetrically flanked by adapter A at one end and adaptor B at the other. These molecules may now be isolated by PS-effect PCR using primers that recognize the outer parts of adapters A and B. Amplification of symmetrically flanked testers from the first stage will be suppressed, and hybrids formed with the driver will also not amplify due to the lack of one or both primer annealing sites, leading to preferential amplification of the asymmetrically flanked sequences of interest.
Mirror Orientation Selection
SSH method has been applied in many handrids of models. Though high efficiency of the technology, unwanted background clones representing non-differentially expressed transcripts can be generated by SSH. First, unincorporated adapters can randomly anneal to non-target cDNA molecules during subtractive hybridization. After DNA elongation, such molecules can serve as a template for amplification and will be picked up in the final product, as will product generated by non-specific annealing of PCR primers.

Answer 1

在第二阶段的减法，样品A和B （有不同的连接适配器）的混合和允许reassociate 。这导致建立新型分子从减去单链DNA的定义是两边不对称适配器阿一端和适配器B作其他。这些分子现在可能被孤立的聚苯乙烯影响PCR扩增引物，承认使用外部适配器A和B扩增测试从两侧对称的第一阶段将抑制，并形成了混合动力汽车的司机也不会因放大缺乏一个或两个引物退火的网站，导致优惠扩增不对称两侧序列的利益。
镜取向选择
SSH的方法已应用于许多handrids模式。虽然高效率的技术，不需要的背景克隆的非差异表达的转录可以生成通过SSH 。首先，非法适配器可以随机退火对非目标基因分子在消减杂交。经过DNA的断裂伸长率，这些分子可以作为模板扩增，将加快最终产品，如将产品所产生的非特异性退火的PCR引物。