找人帮我翻译一下这几段英语,论文就差它们了,不要用软件翻译的,谢谢各位大哥大姐!
2.6.ScreeningforrepellentactivityofplantoilsEssentialoils,whichderivedfromtenplantspe...
2.6. Screening for repellent activity of plant oils
Essential oils, which derived from ten plant species, with and without vanillin, were screened for repellency against
female blood-starved A. aegypti under laboratory conditions. The human-bait method [12], with a slight modification
used to determine the repellency, was conducted from 8.00 to 16.00 h, in a 10×10×3 m room, at 27–35 °C and relative
humidity of 60–80%. Before application of the test samples, the arms of volunteers were washed and cleaned
thoroughly with distilled water. An aliquot (0.1 ml) of each oil sample was applied as uniformly as possible to the skin
exposed through a 30 cm2
hole cut in a plastic sleeve that was wrapped around the ventral part of each forearm. After
applying the sample, volunteers were instructed not to rub, touch or wet the treated forearm. The other forearm, which
acted as a control, was treated with absolute EtOH or 10% vanillin in absolute EtOH by the same procedure as that for
the tested arm. After air drying for 1 min, the treated arm, with gloved hand, was exposed to 250 female mosquitoes in a
cage (30×30×30 cm) for 3 min. The mosquitoes that landed on the tested area and attempted to probe and imbibe
blood were recorded. Exposure experiments continued at 30 min intervals until at least two bites occurred in a 3-min
period, or a first bite occurred followed by a confirming bite (second bite) in the next observation period. During the
experiment, successive introductions of the control arm were performed in the same manner before each insertion of the
treated arm, in order to confirm and standardize the readiness of the mosquitoes to bite. The time between application of
the plant sample and the first two consecutive bites was considered as the complete-protection time, which was the
usual criterion used to determine the repellent efficacy of plant oil. Four replicates were carried out on different days for
each sample and subject. No one tested more than 1 sample per day. The order of tests was assigned randomly and the
volunteers were blinded to the sample used.
2.8.
Qualitative analysis of the chemical constituents in the most effective oil derived from the repellency test was carried
out by GC-MS, recorded at 70 eV, under the following conditions: Alltech 15897 AT-1MS capillary column
(30 m×0.25 mm ID×0.25 μm, film thickness); held at 60 °C for 1 min, raised to 150 °C at a rate of 6 °C/min, raised to
240 °C at a rate of 10 °C/min, and held for 6 min; 250 °C injector temperature; carrier helium at a flow rate of 1.0 ml/
min; 300:1 split ratio. Diluted oil (0.1 μl, 1/10, v/v, in CH2Cl2) was injected automatically into the splitless mode. The
oil components were identified by comparison with standards, by spiking, and on the basis of their mass spectral
fragmentation using the NIST 98 andWiley 275 GC-MS library. Percentage of the identified compound was computed
from a total ion chromatogram (TIC). 展开
Essential oils, which derived from ten plant species, with and without vanillin, were screened for repellency against
female blood-starved A. aegypti under laboratory conditions. The human-bait method [12], with a slight modification
used to determine the repellency, was conducted from 8.00 to 16.00 h, in a 10×10×3 m room, at 27–35 °C and relative
humidity of 60–80%. Before application of the test samples, the arms of volunteers were washed and cleaned
thoroughly with distilled water. An aliquot (0.1 ml) of each oil sample was applied as uniformly as possible to the skin
exposed through a 30 cm2
hole cut in a plastic sleeve that was wrapped around the ventral part of each forearm. After
applying the sample, volunteers were instructed not to rub, touch or wet the treated forearm. The other forearm, which
acted as a control, was treated with absolute EtOH or 10% vanillin in absolute EtOH by the same procedure as that for
the tested arm. After air drying for 1 min, the treated arm, with gloved hand, was exposed to 250 female mosquitoes in a
cage (30×30×30 cm) for 3 min. The mosquitoes that landed on the tested area and attempted to probe and imbibe
blood were recorded. Exposure experiments continued at 30 min intervals until at least two bites occurred in a 3-min
period, or a first bite occurred followed by a confirming bite (second bite) in the next observation period. During the
experiment, successive introductions of the control arm were performed in the same manner before each insertion of the
treated arm, in order to confirm and standardize the readiness of the mosquitoes to bite. The time between application of
the plant sample and the first two consecutive bites was considered as the complete-protection time, which was the
usual criterion used to determine the repellent efficacy of plant oil. Four replicates were carried out on different days for
each sample and subject. No one tested more than 1 sample per day. The order of tests was assigned randomly and the
volunteers were blinded to the sample used.
2.8.
Qualitative analysis of the chemical constituents in the most effective oil derived from the repellency test was carried
out by GC-MS, recorded at 70 eV, under the following conditions: Alltech 15897 AT-1MS capillary column
(30 m×0.25 mm ID×0.25 μm, film thickness); held at 60 °C for 1 min, raised to 150 °C at a rate of 6 °C/min, raised to
240 °C at a rate of 10 °C/min, and held for 6 min; 250 °C injector temperature; carrier helium at a flow rate of 1.0 ml/
min; 300:1 split ratio. Diluted oil (0.1 μl, 1/10, v/v, in CH2Cl2) was injected automatically into the splitless mode. The
oil components were identified by comparison with standards, by spiking, and on the basis of their mass spectral
fragmentation using the NIST 98 andWiley 275 GC-MS library. Percentage of the identified compound was computed
from a total ion chromatogram (TIC). 展开
4个回答
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26。对杀虫剂活性筛选植物精油
精油,来自10种的植物,与无香兰素,并为排斥
女:在实验室条件下blood-starved埃及。这个human-bait方法[12],作了一些修改
用于测定,从上午进行排斥,16.00 h,在10××10个房间,在27-35 300℃和相对
60-80%湿度。申请前的测试样品,手臂的志愿者被清洗干净
彻底地用蒸馏水。一个aliquot(0.1毫升)的油样品用于尽可能统一
通过一个30暴露平方厘米
在一个塑料套筒孔,包裹着腹部分为前臂。之后,
采用样本,志愿者被告知不要擦,触摸或湿的治疗前臂。其他前臂,它
作为控制,是绝对EtOH或10%的绝对EtOH香草的相同的步骤
这个测试的手臂。风干后1分钟,处理,和手套把手臂,暴露于250名女性蚊子在
××笼(30)三十(30)天内,30厘米3分钟。蚊子,落在了测试区,试图探索和吸收水分
血都被记录。在30分钟内暴露实验间隔至少两个咬到3-min发生
期间,或第一口发生,紧随其后的是一个确认咬(二)在下一次咬观察期。期间,
实验,连续引入控制臂进行之前都以相同的方式,
对待手臂,以便确认和规范就绪的蚊子咬。应用之间的时间
植物样品和前两场的complete-protection叮咬被认为是时间,这就是
平时则用来确定排斥的植物精油效果。4重复进行,在不同的日子
下面的自己烦吧
精油,来自10种的植物,与无香兰素,并为排斥
女:在实验室条件下blood-starved埃及。这个human-bait方法[12],作了一些修改
用于测定,从上午进行排斥,16.00 h,在10××10个房间,在27-35 300℃和相对
60-80%湿度。申请前的测试样品,手臂的志愿者被清洗干净
彻底地用蒸馏水。一个aliquot(0.1毫升)的油样品用于尽可能统一
通过一个30暴露平方厘米
在一个塑料套筒孔,包裹着腹部分为前臂。之后,
采用样本,志愿者被告知不要擦,触摸或湿的治疗前臂。其他前臂,它
作为控制,是绝对EtOH或10%的绝对EtOH香草的相同的步骤
这个测试的手臂。风干后1分钟,处理,和手套把手臂,暴露于250名女性蚊子在
××笼(30)三十(30)天内,30厘米3分钟。蚊子,落在了测试区,试图探索和吸收水分
血都被记录。在30分钟内暴露实验间隔至少两个咬到3-min发生
期间,或第一口发生,紧随其后的是一个确认咬(二)在下一次咬观察期。期间,
实验,连续引入控制臂进行之前都以相同的方式,
对待手臂,以便确认和规范就绪的蚊子咬。应用之间的时间
植物样品和前两场的complete-protection叮咬被认为是时间,这就是
平时则用来确定排斥的植物精油效果。4重复进行,在不同的日子
下面的自己烦吧
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这么多啊,人工的是要成本的啊 要钱的哦。
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这么繁重的活都没赏金啊。。
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